SMAC/Diablo Antibody [Unconjugated]

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, CHP-100 human neuroblastoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human SMAC/Diablo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF789) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Recombinant Human SMAC/Diablo (Catalog # 789-SM) (0.5 ng/lane) was included. A specific band for SMAC/Diablo was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.

Simple Western lane view shows lysates of SH‑SY5Y human neuroblastoma cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for SMAC/Diablo at approximately 29 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human SMAC/Diablo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF789). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

SMAC/Diablo Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human SMAC/Diablo
  Ala56-Asp239
  Accession # Q9NR28
• Specificity: Detects human SMAC/Diablo in Western blots. Also detects mouse and rat SMAC/Diablo when the salt concentration of blotting buffer is reduced to 0.15 M NaCl, and 1 μg/mL anti-SMAC/Diablo concentration is used.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DIABLO
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SMAC/Diablo Antibody [Unconjugated]

• 0610041G12Rik
• Diablo
• diablo, IAP-binding mitochondrial protein
• Direct IAP-binding protein with low pI
• mitochondrial Smac protein
• Second mitochondria-derived activator of caspase
• SMAC
• SMACmitochondrial

Background

SMAC (second mitochondria derived activator of caspase)/Diablo promotes caspase activation by interacting with the inhibitor of apoptosis (IAP) proteins in the cytochrome c/Apaf-1/caspase-9 pathway.

1. Du, C. et al. (2000) Cell 102:33.
2. Chai, J. et al. (2000) Nature 406:855.
3. Srinivasula, S. et al. (2000) J. Biol. Chem. 275:36152.
4. Ekert, P. et al. (2001) J. Cell Biol. 152:483.
5. Verhagen, A. (2000) Cell 102:43.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SMAC/Diablo Antibody (AF789)(9)

Publications using AF789

• Xue‑Jiao Sun, Zhan‑Hua Li, Yang Zhang, Xiao‑Ning Zhong, Zhi‑Yi He, Ji‑Hong Zhou et al. Theophylline and dexamethasone in combination reduce inflammation and prevent the decrease in HDAC2 expression seen in monocytes exposed to cigarette smoke extract Experimental and Therapeutic Medicine 3/6/2020 [PMID: 32269608]
• Xue‑Jiao Sun, Zhan‑Hua Li, Yang Zhang, Xiao‑Ning Zhong, Zhi‑Yi He, Ji‑Hong Zhou et al. Theophylline and dexamethasone in combination reduce inflammation and prevent the decrease in HDAC2 expression seen in monocytes exposed to cigarette smoke extract Experimental and Therapeutic Medicine 2020-03-06 [PMID: 32269608] (Western Blot, Human)
• Pro-Apoptotic Activity of New Honokiol/Triphenylmethane Analogues in B-Cell Lymphoid Malignancies Molecules, 2016-07-30;21(8):. 2016-07-30 [PMID: 27483232] (Flow Cytometry, Human)
• L. Flanagan, J. Kehoe, J. Fay, O. Bacon, A.U. Lindner, E.W. Kay et al. High levels of X-linked Inhibitor-of-Apoptosis Protein (XIAP) are indicative of radio chemotherapy resistance in rectal cancer Radiation Oncology 12/1/2015 [PMID: 26071313]
• L. Flanagan, J. Kehoe, J. Fay, O. Bacon, A.U. Lindner, E.W. Kay et al. High levels of X-linked Inhibitor-of-Apoptosis Protein (XIAP) are indicative of radio chemotherapy resistance in rectal cancer Radiation Oncology 2015-12-01 [PMID: 26071313] (Western Blot, Human)
• E Passante, M L Würstle, C T Hellwig, M Leverkus, M Rehm Systems analysis of apoptosis protein expression allows the case-specific prediction of cell death responsiveness of melanoma cells Cell Death & Differentiation 11/1/2013 [PMID: 23933815]
• Laussmann MA, Passante E, Hellwig CT Proteasome inhibition can impair caspase-8 activation upon submaximal stimulation of apoptotic tumor necrosis factor-related apoptosis inducing ligand (TRAIL) signaling. J. Biol. Chem., 2012-03-09;287(18):14402-11. 2012-03-09 [PMID: 22408249] (Western Blot, Human)
• Wang H, Jiang JY, Zhu C, Peng C, Tsang BK Role and regulation of nodal/activin receptor-like kinase 7 signaling pathway in the control of ovarian follicular atresia. Mol. Endocrinol., 2006-05-18;20(10):2469-82. 2006-05-18 [PMID: 16709598] (Western Blot, Rat)
• You RI, Chou YC Inhibition of lymphotoxin-beta receptor-mediated cell death by survivin-DeltaEx3. Cancer Res., 2006-03-15;66(6):3051-61. 2006-03-15 [PMID: 16540654] (ICC, Human)

Bioinformatics

• Gene Symbol: DIABLO
• Uniprot:
  • Q9NR28()