SMAC/Diablo Antibody (78-1-118)

Images

 
Western Blot: Smac Antibody (78-1-118) [NB500-213] - HeLa whole cell extract lysates.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IP
Clone
78-1-118
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

SMAC/Diablo Antibody (78-1-118) Summary

Immunogen
Recombinant mature human Smac (lacks amino acids 1-55 present in Smac precursor). [UniProt# Q9NR28]
Localization
Mitochondrial but released into the cytosol when cells undergo apoptosis.
Isotype
IgG3/IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DIABLO
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation 1:10-1:500
Application Notes
This Smac (78-1-118) antibody is useful for Immunoprecipitation and Western Blot, where a band is seen at ~25kDa. For better results IP prior to Western blot is recommended.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
293 Lysate (NB800-PC6)
Publications
Read Publications using
NB500-213 in the following applications:

Reactivity Notes

Human, rat, mouse.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for SMAC/Diablo Antibody (78-1-118)

  • 0610041G12Rik
  • Diablo
  • diablo, IAP-binding mitochondrial protein
  • Direct IAP-binding protein with low pI
  • mitochondrial Smac protein
  • Second mitochondria-derived activator of caspase
  • SMAC
  • SMACmitochondrial

Background

Smac/DIABLO, a mitochondrial protein, activates various forms of apoptosis. This activation may be due to the neutralization of one or more members of the IAP family of apoptosis inhibitory proteins. Smac exits the mitochondria and enter the cytosol during certain apoptosis triggered events. Mitochondrial-mediated apoptosis is important in animal development and tissue homeostasis, with alterations resulting in a range of malignant disorders. Upon apoptotic stimuli, the mitochondrial proteins cytochrome c and Smac/DIABLO are released into the cytosol. The release of these proteins, however, occurs via different mechanisms (2,3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SMAC/Diablo Antibody (NB500-213)(9)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(6)
All Applications
Filter By Species
Mouse
(6)
Rat
(2)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB500-213 Applications Species
Hwang HJ, Dornbos P, Steidemann M et al. Mitochondrial-targeted aryl hydrocarbon receptor and the impact of 2,3,7,8-tetrachlorodibenzo-p-dioxin on cellular respiration and the mitochondrial proteome. Toxicol. Appl. Pharmacol. 2016 Apr 20 [PMID: 27105554] (WB, Mouse) WB Mouse
Sirianni AC, Jiang J, Zeng J et al. N-acetyl-L-tryptophan, but not N-acetyl-D-tryptophan, Rescues Neuronal Cell Death in Models of Amyotrophic Lateral Sclerosis J. Neurochem. 2015 May 30 [PMID: 26031348] (WB, Mouse) WB Mouse
Zhang Y, Chen LY, Han X et al. Melatonin inhibits the caspase-1/cytochrome c/caspase-3 cell death pathway, inhibits MT1 receptor loss and delays disease progression in a mouse model of amyotrophic lateral sclerosis Neurobiol Dis 2013 Mar 26 [PMID: 23537713] (WB, Mouse) WB Mouse
Wang X, Sirianni A, Pei Z et al. The Melatonin MT1 Receptor Axis Modulates Mutant Huntingtin-Mediated Toxicity. The Journal of Neuroscience: The Official Journal of the Society for Neuroscience;31(41):14496-14507. 2011 Oct 12. [PMID: 21994366]
Wang X et al. Inhibitors of cytochrome c release with therapeutic potential for Huntington's disease. J Neurosci;28(38):9473-85. 2008 Sep 17. [PMID: 18799679] (WB, Mouse, Rat) WB Mouse, Rat
Zhang, WH et al. Nortriptyline protects mitochondria and reduces cerebral ischemia/hypoxia injury. Stroke;39(2):455-62. 2008 Feb. [PMID: 18174477]
Liu, JC et al. Identification of tumorsphere-and tumor-initiating cells in HER2/Neu-induced mammary tumors. Cancer Res;67(18):8671-81. 2007 Sep 15. [PMID: 17875707]
Zhang, WH et al. Fundamental role of the Rip2/caspase-1 pathway in hypoxia and ischemia-induced neuronal cell death. Proc Natl Acad Sci U S A;100(26):16012-7. 2003 Dec 23. [PMID: 14663141] (WB, Mouse) WB Mouse
Wang, X et al. Minocycline inhibits caspase-independent and -dependent mitochondrial cell death pathways in models of Huntington's disease. Proc Natl Acad Sci U S A;100(18):10483-7. 2003 Sep 2. [PMID: 12930891] (WB, ICC/IF, Mouse, Rat) WB, ICC/IF Mouse, Rat

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Product General Protocols

View specific protocols for SMAC/Diablo Antibody (NB500-213): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SMAC/Diablo Antibody (NB500-213) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for SMAC/Diablo Antibody (NB500-213)

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Blogs on SMAC/Diablo.

Apoptosis and Necroptosis Part II: Inhibitors of apoptosis proteins (IAPs); Key regulators of the balance between necroptosis, apoptosis and survival
In the first installment of this two-part blog post titled "Apoptosis and Necroptosis: Important factors to identify both types of programmed cell death", the mechanisms by which cell death occurs and ways to identify these pathways were...  Read full blog post.

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Bioinformatics

Gene Symbol DIABLO
Entrez
Uniprot