Slit2 Antibody

Images

 
Western Blot: Slit2 Antibody [NBP2-20398] - Various tissue extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SLIT2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody ...read more
Immunohistochemistry-Paraffin: Slit2 Antibody [NBP2-20398] - Paraffin-embedded mouse fetal brain. Green: SLIT2 antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488). Blue: ...read more
Western Blot: Slit2 Antibody [NBP2-20398] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SLIT2 antibody diluted at 1:1000.
Western Blot: Slit2 Antibody [NBP2-20398] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SLIT2 antibody diluted at 1:1000.
Western Blot: Slit2 Antibody [NBP2-20398] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SLIT2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody ...read more
Immunohistochemistry-Paraffin: Slit2 Antibody [NBP2-20398] - SLIT2 Antibody [NBP2-20398] - Cytosol and membrane on gastric carcinoma.
Immunohistochemistry-Paraffin: Slit2 Antibody [NBP2-20398] - SLIT2 antibody detects SLIT2 protein at cytoplasm in rat brain Paraffin-embedded rat brain diluted at 1:400.
Immunoprecipitation: Slit2 Antibody [NBP2-20398] - Raji whole cell lysate/extract A : 30 ug whole cell lysate/extract of SLIT2 protein expressing Raji cells B : Control with 3 ug of pre-immune rabbit IgG C : ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, XpSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Slit2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SLIT2. The exact sequence is proprietary.
Localization
Secreted
Predicted Species
Bovine (99%), Xenopus (93%), Chicken (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLIT2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:10000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
170 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Slit2 Knockout 293T Cell Lysate
Publications
Read Publication using
NBP2-20398 in the following applications:

  • WB
    1 publication

Reactivity Notes

Zebrafish (88%), Xenopus laevis (93%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for Slit2 Antibody

  • FLJ14420
  • SLIL3
  • slit (Drosophila) homolog 2
  • slit homolog 2 (Drosophila)
  • slit homolog 2 protein
  • Slit2
  • Slit-2SLIL3

Background

Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Slit2 Antibody (NBP2-20398)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SLIT2
Uniprot