Slit2 Antibody

Images

 
Western Blot: Slit2 Antibody [NB600-881] - Analysis of a band at 165 kDa (lane 1) corresponding to SLIT-2 present in a chicken spinal cord whole cell lysate (arrowhead). Approximately 30 ug of lysate was separated on a ...read more
Immunohistochemistry: Slit2 Antibody [NB600-881] - Analysis of human breast carcinoma Tissue: Human prostate Fixation: FFPE buffered formalin 10% conc Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker, EDTA pH 9.5 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, XpSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Slit2 Antibody Summary

Immunogen
Synthetic peptide: YFIPGTEDYRSKLSGDC conjugated to KLH, corresponding to amino acids 484-500 of Human SLIT2.
Localization
Secreted
Specificity
Expect a band at ~ 165 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLIT2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:1500-1:6000
  • Immunohistochemistry 1:10-1:500
Application Notes
This affinity purified antibody has been tested for use in ELISA and by western blot. Expect a band at ~ 165 kDa corresponding - full-length protein by western blotting in the appropriate cell lysate or extract. For western blotting block the membrane with goat serum. Do not block with non-fat dry milk.

Reactivity Notes

Known reactivity: human and chicken. Expect cross reactivity with SLIT-2 from Tetraodon nigroviridis, as only a single amino acid residue change is found within the immunogen sequence (94% positive by BLAST). The core 13 amino acids of the immunogen are also 100% homologous to SLIT-2 from mouse, rat, bovine and Xenopus laevis. Cross reactivity with SLIT-2 homologues from other sources has not been determined.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Slit2 Antibody

  • FLJ14420
  • SLIL3
  • slit (Drosophila) homolog 2
  • slit homolog 2 (Drosophila)
  • slit homolog 2 protein
  • Slit2
  • Slit-2SLIL3

Background

Slit proteins are ligands at the Roundabout (Robo) receptors and act as guidance cues in axonal migration/navigation during neural development, at the ventral midline of the neural tube. Slit1 and Slit2 are essential for midline guidance in the forebrain by acting as repulsive signals preventing inappropriate midline crossing by axons projecting from the olfactory bulb. A number of cleavage products are reported in the literature for Slit2 protiens (following alternate splicing). The C-terminal cleavage proteins are more diffusible than the larger N-terminal protein that is more tightly cell associated. Slit2 protein is expressed in fetal lung and kidney, and adult spinal cord. Weak expression in adult adrenal gland, thyroid, trachea.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF3629
Species: Hu, Mu, Rt
Applications: WB, Neut
6514-SL
Species: Hu
AF1749
Species: Rt
Applications: WB, IHC
AF3147
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NB110-58778
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB200-106
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
AF3155
Species: Mu
Applications: WB, IHC
AF844
Species: Mu
Applications: WB, Block
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-87076
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-14907
Species: Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-03644
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-27109
Species: Hu, Mu, Po, Pm
Applications: WB, Simple Western, IHC, IHC-P
NB100-56883
Species: Hu
Applications: IHC, IHC-P
NBP2-46403
Species: Hu
Applications: IHC
NBP1-33116
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Publications for Slit2 Antibody (NB600-881) (0)

There are no publications for Slit2 Antibody (NB600-881).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Slit2 Antibody (NB600-881) (0)

There are no reviews for Slit2 Antibody (NB600-881). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Slit2 Antibody (NB600-881) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Slit2 Products

Bioinformatics Tool for Slit2 Antibody (NB600-881)

Discover related pathways, diseases and genes to Slit2 Antibody (NB600-881). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Slit2 Antibody (NB600-881)

Discover more about diseases related to Slit2 Antibody (NB600-881).
 

Pathways for Slit2 Antibody (NB600-881)

View related products by pathway.

PTMs for Slit2 Antibody (NB600-881)

Learn more about PTMs related to Slit2 Antibody (NB600-881).
 

Research Areas for Slit2 Antibody (NB600-881)

Find related products by research area.

Blogs on Slit2

There are no specific blogs for Slit2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Slit2 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol SLIT2
Entrez