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SLC7A10 Antibody

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Western Blot: SLC7A10 Antibody [NBP3-12252] - WB of NBP3-12252 with SKNMC cells. 1:500 antibody dilution in DiluObuffer.
Western Blot: SLC7A10 Antibody [NBP3-12252] - WB of NBP3-12252 with SKNMC cells. 1:500 antibody dilution in DiluObuffer.
Immunohistochemistry-Paraffin: SLC7A10 Antibody [NBP3-12252] - Mouse Brain- NBP3-12252. 1:50 dilution inIHC Blocking Buffer. DAB (brown) straining and Hematoxylin QS (blue) counterstrain. 40x magnification. FFPE ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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SLC7A10 Antibody Summary

Immunogen
Synthetic peptide taken within amino acid region 1-50 on rat Asc-type amino acid transporter 1 protein.
Clonality
Polyclonal
Host
Rabbit
Gene
SLC7A10
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA 1:4000
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:200
  • Western Blot 1:500-1:100

Packaging, Storage & Formulations

Storage
Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Contains Tris, HCl/glycine buffer (pH 7.4-7.8), 30% glycerol, 0.5% BSA, along with cryo-protective agents, and HEPES (exact storage and stabilization buffer information is proprietary).
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for SLC7A10 Antibody

  • ASC1
  • asc-1
  • asc-type amino acid transporter 1
  • FLJ20839
  • HASC-1
  • solute carrier family 7 (neutral amino acid transporter light chain, asc system), member 10
  • solute carrier family 7 member 10
  • solute carrier family 7, (cationic amino acid transporter, y+ system) member 10
  • solute carrier family 7, (neutral amino acid transporter, y+ system) member 10

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: ASC/TMS1 Antibody [NBP1-78977] - Tested in MCF-7 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunocytochemistry/Immunofluorescence: ASC/TMS1 Antibody [NBP1-78977] - NLRP3 activation was evaluated by visualizing the co-localization of NLRP3 and apoptosis-associated speck-like protein (ASC) using immunocytochemistry (A). PLoS One. 2020 Jun 17;15(6):e0234039. doi: 10.1371/journal.pone.0234039. (Catalog # NBP1-78977AF647)
Immunohistochemistry: ASC/TMS1 Antibody [NBP1-78977] - Rats were infected by S. pneumoniae or given PBS sham control. 21.5 umoles/kg Mito-Vit-E (MVE) or vehicle was administered orally 30 minutes post-inoculation, and heart tissues were harvested 24 hours later. Heart sections were co-stained with anti-ASC (brown) and haematoxylin (blue). Negative control was stained with secondary antibody alone. Images are representative of a random selection of at least 3 sections of N = 6. PLoS One. 2015 Oct 8;10(10):e0139416. doi: 10.1371/journal.pone.0139416.
(2 Reviews)
(28 Publications)
Species: Hu, Mu, Rt
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-P, IM, IP, Simple Western, WB
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NBP1-90095
Immunohistochemistry-Paraffin: Steroid sulfatase Antibody [NBP1-90095] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: Steroid sulfatase Antibody [NBP1-90095] - Staining in human placenta and pancreas tissues using anti-STS antibody. Corresponding STS RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Steroid sulfatase Antibody [NBP1-90095] - Staining of human placenta shows high expression.
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RACK1/GNB2L1
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Flow Cytometry: RACK1/GNB2L1 Antibody (2D8) [NBP2-00612] - Analysis of Hela cells, using anti-GNB2L1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: RACK1/GNB2L1 Antibody (2D8) [NBP2-00612] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GNB2L1 antibody, and then analyzed by flow cytometry.
Immunohistochemistry-Paraffin: RACK1/GNB2L1 Antibody (2D8) [NBP2-00612] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GNB2L1 mouse monoclonal antibody.
Species: Hu
Applications: Flow, IHC, IHC-P, WB
ASC1 Antibody
NBP1-89656
Western Blot: ASC1 Antibody [NBP1-89656] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: ASC1 Antibody [NBP1-89656] - Staining of human colon shows cytoplasmic positivity in glandular cells.
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(1C11.7E3) - Azide an ...
NBP2-80662
Western Blot: CD98 Antibody (1C11.7E3) - Azide and BSA Free [NBP2-80662] - Western blot analysis of human CD98 in recombinant protein, A431, Jurkat, HeLa and HepG2 lysates at 2.0 ug/ml. Image from the standard format of this antibody.
Flow (Cell Surface): CD98 Antibody (1C11.7E3) - Azide and BSA Free [NBP2-80662] - Flow cytometry analysis of human CD98 in hPBMC. Primary antibody was used at 2.5 ug / million cells. Goat anti-mouse IgG (PE) was used at 0.5 ug / million cells. The red shift line represents the primary antibody, the blue line represents the cells alone and the green line represents the isotype control. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: CD98 Antibody (1C11.7E3) - Azide and BSA Free [NBP2-80662] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human kidney using CD98 antibody (clone 1C11.7E3) at 1:300 dilution. The antibody generated a distinct/intense membranous staining with relatively weaker cytoplasmic signal, and the immuno
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Western Blot: ASC2 Antibody [NB100-56076] - Used to analyze ASC2/POP1 . HEK-293 cells were transiently transfected with plasmids encoding ASC-2, POP1 or a negative control plasmid. ASC-2 and POP1 were detected at the expected 12 kDa molecular weight.
Immunohistochemistry: ASC2 Antibody [NB100-56076] - Used at 1:2000 to analyze ASC-2/POP1 expression in mouse. A) prostate tissue from a male mouse prostatitis. B) kidney from a mouse with pyelonephritis. A1 and B1, high magnification of A and B, respectively.
Immunohistochemistry-Paraffin: ASC2 Antibody [NB100-56076] - Used at 1:2000 to analyze ASC-2 expression in mouse spleen. A and A1, spleen at low and high magnification, respectively. ASC-2 staining is seen in the granulocytes, whereas the lymph and megakaryocytes are negative.
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Immunohistochemistry: SLC7A9 Antibody [NBP2-93638] - Analysis of human kidney cancer using SLC7A9 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry: SLC7A9 Antibody [NBP2-93638] - Analysis of human kidney cancer using SLC7A9 at dilution of 1:100. Blue: DAPI for nuclear staining.
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NBP1-89327
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Immunohistochemistry-Paraffin: SLC1A5 Antibody [NBP1-89327] - Staining of human prostate shows strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: SLC1A5 Antibody [NBP1-89327] - Staining of human rectum shows moderate to strong membranous positivity in glandular cells.
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Androgen R/NR3C4
[p Ser213, p Ser210] ...
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Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on HeLa cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650.
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Human, Mouse, Rat
RelA/NFkB p65 ELISA K ...
NBP2-29661
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ELISA: Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric) [NBP2-29661] - Standard curve using the internal RelA standard.
ELISA: Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric) [NBP2-29661] - Nuclear translocation of NF-kB p65 with LPS in Jurkat cells. Jurkat cells, grown to a density of 2X10(6) cells/ml, were treated with LPS (15 ug/ml). The cells were harvested at various indicated time points, and cell lysates were tested in Western analysis using NB100-56712 and also in this ELISA Kit.
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BBS9 Antibody
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Immunohistochemistry-Paraffin: BBS9 Antibody [NBP1-88927] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
(3 Publications)
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DAO Antibody
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Immunocytochemistry/Immunofluorescence: DAO Antibody [NBP2-92892] - Analysis of L929 cells using DAO at dilution of 1:100. Blue: DAPI for nuclear staining.
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[Unconjugated]
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Immunocytochemistry/Immunofluorescence: GRASP65 Antibody (OTI5G8) [NBP2-02665] - Detection in cultured pig trabecular meshwork cells. Image from a verified customer review.
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(3 Reviews)
(2 Publications)
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Western Blot: SLC3A1 Antibody [NBP2-47272] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human plasma (IgG/HSA depleted)
Immunohistochemistry-Paraffin: SLC3A1 Antibody [NBP2-47272] - Staining in human kidney and cervix, uterine tissues using anti-SLC3A1 antibody. Corresponding SLC3A1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SLC3A1 Antibody [NBP2-47272] - Staining of human cervix, uterine shows low expression as expected.
Species: Hu
Applications: IHC, IHC-P, WB
NCOA6 Antibody
NB200-335
Western Blot: NCOA6 Antibody [NB200-335] - Detection of Human ASC2 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASC2 antibody NB200-335 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: NCOA6 Antibody [NB200-335] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ASC2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: NCOA6 Antibody [NB200-335] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASC2 antibody used for IP at 6 ug per reaction. ASC2 was also immunoprecipitated by a previous lot of this antibody and rabbit anti-ASC2 antibody. For blotting immunoprecipitated ASC2, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
(7 Publications)
Species: Hu
Applications: IHC, IHC-P, IP, WB

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Bioinformatics

Gene Symbol SLC7A10