ASC2 Antibody

Images

 
Western Blot: ASC2 Antibody [NB100-56076] - Used to analyze ASC2/POP1. HEK-293 cells were transiently transfected with plasmids encoding ASC-2, POP1 or a negative control plasmid. ASC-2 and POP1 were detected at the ...read more
Immunohistochemistry-Paraffin: ASC2 Antibody [NB100-56076] - Used at 1:2000 to analyze ASC-2 expression in mouse spleen. A and A1, spleen at low and high magnification, respectively. ASC-2 staining is seen in the ...read more
Immunohistochemistry: ASC2 Antibody [NB100-56076] - Used at 1:2000 to analyze ASC-2/POP1 expression in mouse. A) prostate tissue from a male mouse prostatitis. B) kidney from a mouse with pyelonephritis. A1 and B1, high ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ASC2 Antibody Summary

Immunogen
A recombinant full-length human ASC-2/POP1 protein was used as immunogen.
Specificity
In immuhistochemical studies, ASC-2/POP1 has been found to expressed predominantly in macrophages and granulocytes (Stehlik et al, 2003).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PYDC1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
Control
Human Spleen Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publication using NB100-56076.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Neat whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for ASC2 Antibody

  • ASC2PAAD-only protein 1
  • ASCI
  • POP1pyrin domain-containing protein 1
  • PYC1
  • PYD (pyrin domain) containing 1
  • pyrin domain containing 1
  • pyrin-domain containing protein 1
  • Pyrin-only protein 1

Background

ASC-2/POP1 is a PAAD domain only protein originally identified in a bioinformatics screen aimed at understanding molecular apoptosis mechanisms (Pawtokski et al, 2001; reviewed in Fabiola et al, 2006, and Mariathasan and Vuvic, 2003.). ASC-2/POP1 has high amino acid sequence homology with ASC (64%), hence it was originally termed ASC-2. The PAAD (also known as PYRIN) domain is a conserved sequence motif identified in more than 35 human proteins with putative functions in apoptosis and inflammatory signaling pathways. PAAD was named after the protein families from which it was discovered: pyrin, AIM (absent-in-melanoma), ASC [apoptosis-associated speck-like protein containing a caspase recruitment domain (CARD], and death-domain (DD)-like. PAAD is thought to function as a protein-protein interaction domain, possibly coupling different signaling pathways such as apoptosis, inflammation and cancer. For example, PAADs are capable of homotypic interactions with themselves or other members of the PAAD family, suggesting that they participate in complex protein-interaction networks that link various signaling pathways. In humans, the gene encoding ASC-2/POP1 is on chromosome 16p12.1, only 14 kbp away from the ASC locus. The proximity of ASC-2/POP1 to ASC as well as the high sequence homology between them suggest that the ASC-2/POP1 and ASC genes arose by gene duplication. Studies have shown that ASC-2/POP1 associates with ASC via PAAD-PAAD interactions and modulates ASC-mediated roles in apoptosis and inflammation. ASC-2/POP1 may also have a role in modulating other multidomain PAAD-containing proteins. However, the physiological relevance of ASC-2/POP1 remains to be fully elucidated. Human ASC-2/POP1 is an 89 amino acid protein and migrates at ~10-12 kDa on SDS-PAGE gels (Stehlik et al, 2003).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ASC2 Antibody (NB100-56076)(1)

We have publications tested in 1 application: WB.


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Bioinformatics

Gene Symbol PYDC1
Entrez