SLC6A3/DAT1 Antibody

Images

 
Western Blot: DAT1 Antibody [NB300-255] - Detection of DAT protein in human caudate sample using NB 300-255.
Western Blot: DAT1 Antibody [NB300-255] - Western blot analysis of DAT1 in SDS-solubilized human caudate using NB300-255 at 1:1000.

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, IHC, IHC-Fr
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.3 mg/ml

Order Details

SLC6A3/DAT1 Antibody Summary

Immunogen
Peptide from the extracellular loop 2 (EL2) region of human dopamine transporter (DAT), conjugated to keyhole limpet hemocyanin (KLH).
Specificity
Specific for the ~88k DAT protein in Western blots.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC6A3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:1000

Reactivity Notes

The antibody has been tested in Western blots of SDS-solubilized human striatal samples and in IHC applications with formaldehyde-fixed human and monkey brain sections.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
0.3 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SLC6A3/DAT1 Antibody

  • DA transporter
  • DAT1
  • DATDAT1Solute carrier family 6 member 3
  • SLC6A3
  • sodium-dependent dopamine transporter
  • solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
  • VNTR

Background

Plasmalemmal neurotransmitter transporters sequester synaptic and peri-synaptic transmitter into presynaptic elements. The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. Levels of DAT protein expression are altered by chronic drug administration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00006532-M06
Species: Hu
Applications: ELISA, IP
NB300-109
Species: Hu, Mu, Rt, Dr, I, Ma
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl
H00001815-R02
Species: Hu
NBP1-60120
Species: Hu
Applications: WB, TCS
NBP1-85818
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-69750
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-00178
Species: Mu
Applications: WB, IHC, IHC-Fr, IHC-P, PEP-ELISA
NBP1-31386
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-02398
Species: Hu, Mk
Applications: ELISA, IHC, IHC-P
248-BD/CF
Species: Hu, Mu, Rt, Ca, Eq
NBP1-88927
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-89396
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-01752
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-26380
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB110-60019
Species: Mu, Rt, Hu(-)
Applications: WB, ICC/IF, IHC
AF2156
Species: Hu, Mu
Applications: WB, IHC
NB600-534
Species: Hu, Po, Vi
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, RIA
NB300-255
Species: Hu, Pm
Applications: WB, IHC, IHC-Fr

Publications for SLC6A3/DAT1 Antibody (NB300-255) (0)

There are no publications for SLC6A3/DAT1 Antibody (NB300-255).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SLC6A3/DAT1 Antibody (NB300-255) (0)

There are no reviews for SLC6A3/DAT1 Antibody (NB300-255). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SLC6A3/DAT1 Antibody (NB300-255). (Showing 1 - 1 of 1 FAQ).

  1. Could you kindly provide published references for Western blot in detection of Dopamine transporter in human brain tissue with your antibody/antibodies?
    • Catalog numbers NBP2-22164, NB300-254 and NB100-65459 are well characterized DAT1 antibodies that have been referenced. If you refer to our website we have links to the PMID listing for each reference on the individual datasheets.

Secondary Antibodies

 

Isotype Controls

Additional SLC6A3/DAT1 Products

Bioinformatics Tool for SLC6A3/DAT1 Antibody (NB300-255)

Discover related pathways, diseases and genes to SLC6A3/DAT1 Antibody (NB300-255). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SLC6A3/DAT1 Antibody (NB300-255)

Discover more about diseases related to SLC6A3/DAT1 Antibody (NB300-255).
 

Pathways for SLC6A3/DAT1 Antibody (NB300-255)

View related products by pathway.

PTMs for SLC6A3/DAT1 Antibody (NB300-255)

Learn more about PTMs related to SLC6A3/DAT1 Antibody (NB300-255).
 

Research Areas for SLC6A3/DAT1 Antibody (NB300-255)

Find related products by research area.

Blogs on SLC6A3/DAT1

There are no specific blogs for SLC6A3/DAT1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SLC6A3/DAT1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol SLC6A3
Entrez
Uniprot