SLC6A3/DAT1 Antibody - Azide Free

Western Blot: SLC6A3/DAT1 Antibody [NB300-254] - Western blot of human caudate lysate showing specific immunolabeling of the ~88k DAT protein.

Western Blot: SLC6A3/DAT1 Antibody [NB300-254] - Detection of Dopamine Transporter in human caudate lysate using NB 300-254.

• Reactivity: Hu, Mu, Rt, Ha, Pm, SyHa
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

SLC6A3/DAT1 Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit SLC6A3/DAT1 Antibody - Azide Free (NB300-254) is a polyclonal antibody validated for use in IHC and WB. Anti-SLC6A3/DAT1 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to amino acid residues from the intracellular C-terminal region conjugated to KLH. Accession # Q01959
• Localization: Integral membrane protein.
• Specificity: Specific for endogenous levels of the ~80 kDa DAT protein. Diffuse band may be observed between ~70-85 kDa due to variable glycosylation.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC6A3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:1000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:1000
• Application Notes: Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 22357095)
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Hamster reported in lierature (PMID: 22357095). Golden Syrian Hamster reactivity reported in scientific literature (PMID: 22357095).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for SLC6A3/DAT1 Antibody - Azide Free

• DA transporter
• DAT1
• DATDAT1Solute carrier family 6 member 3
• SLC6A3
• sodium-dependent dopamine transporter
• solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
• VNTR

Background

The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SLC6A3/DAT1 Antibody (NB300-254)(4)

Publications using NB300-254

• Nguyen, MT;Vemaraju, S;Nayak, G;Odaka, Y;Buhr, ED;Alonzo, N;Tran, U;Batie, M;Upton, BA;Darvas, M;Kozmik, Z;Rao, S;Hegde, RS;Iuvone, PM;Van Gelder, RN;Lang, RA; An opsin 5-dopamine pathway mediates light-dependent vascular development in the eye Nat. Cell Biol. 2019-04-01 [PMID: 30936473] (WB, Mouse)
• Xue X, Yang JY, He Y et al. Aggregated single-walled carbon nanotubes attenuate the behavioural and neurochemical effects of methamphetamine in mice. Nat Nanotechnol 2016-01-01 [PMID: 26974957] (WB, Mouse)
• Savolainen MH, Richie CT, Harvey BK et al. The beneficial effect of a prolyl oligopeptidase inhibitor, KYP-2047, on alpha-synuclein clearance and autophagy in A30P transgenic mouse. Neurobiol. Dis. 2014-04-16 [PMID: 24746855] (WB, IHC-Fr, Mouse)
• Rodriguez S, Uchida K, Nakayama H. Immunohistochemical changes of nigrostriatal tyrosine hydroxylase and dopamine transporter in the golden hamster after a single intrastriatal injection of 6-hydroxydopamine. Exp Toxicol Pathol. 2012-02-20 [PMID: 22357095] (IHC-P, Golden Syrian Hamster)

FAQs for SLC6A3/DAT1 Antibody (NB300-254). (Showing 1 - 2 of 2 FAQs).

1. Could you kindly provide published references for Western blot in detection of Dopamine transporter in human brain tissue with your antibody/antibodies?
   • Catalog numbers NBP2-22164, NB300-254 and NB100-65459 are well characterized DAT1 antibodies that have been referenced. If you refer to our website we have links to the PMID listing for each reference on the individual datasheets.
2. Our customer would like to know if NB300-254 can be used with samples from rat, since it can recognize mouse and the similarity reaches 99%.
   • NB300-254 has not been tested or validated for use in rat and is therefore not covered by our guarantee.

Bioinformatics

• Gene Symbol: SLC6A3
• Entrez:
  • 6531(Human)
  • 13162(Mouse)
• Uniprot:
  • Q01959(Human)