SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free

Western Blot: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - Analysis of SLC31A1/CTR1 in MCF7 and HeLa at 2 ug/ml.

Immunocytochemistry/Immunofluorescence: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti SLC31A1/CTR1 (5E6.1B4) NBP2-36573 at a 1:50 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - IL-17 induces cellular copper uptake through induction of STEAP4. Western blots analysis for copper trafficking-associated proteins from lysates of mouse colon epithelial organoids treated with different inflammatory cytokines for 8h. Three independent experiments were done and representative blots were shown. Untr untreated. IL-17 primed or unprimed mouse colon organoids were stained for STEAP4 (St4), E-cadherin (E-cad) and SLC31A1/CTR1 and integrin alpha. Scale bar, 100 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-020-14698-y), licensed under a CC-BY license.

Western Blot: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - STEAP4 promotes colitis-associated colon tumorigenesis. Western blot analysis of colon tissue lysates from mice treated with DSS water for indicated days. Western blot analysis of colon tissue lysates from mice treated with DSS water for indicated days. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32060280/) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - Analysis of a formalin fixed and paraffin embedded tissue section of human urinary bladder transitional cell carcinoma (NBP2-30234) using purified CTR1/SLC31A1 antibody clone 5E6.1B4 at 5 ug/ml concentration.

Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - Analysis of a FFPE tissue section of normal breast tissue from a human breast cancer patient (NBP2-30234) using purified CTR1/SLC31A1 antibody clone 5E6.1B4 at 5 ug/ml concentration. The antibody generated a very strong cytoplasmic staining in the myo-epithelial cells in the tested section.

Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - Analysis of a FFPE tissue section of normal skin from a human breast cancer patient (NBP2-30234) using purified CTR1/SLC31A1 antibody clone 5E6.1B4 at 5 ug/ml concentration. The antibody developed a strong cytoplasmic staining in the outer layer of stratum spinosum and the cells of stratum granulosum/ granular layer of skin epidermis. The endothelial cells in the dermis of the section also showed positivity for SLC31A1/CTR1.

Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - Aanalysis of a FFPE tissue section of human small bowel malignant stromal tumor (NBP2-30234) using purified CTR1/SLC31A1 antibody clone 5E6.1B4 at 5 ug/ml concentration. The cancer cells developed moderate to strong positivity for CTR1/SLC31A1 protein.

• Reactivity: Hu, Mu, Rt, Po, Pm
• Applications: WB, ICC/IF, IHC
• Clone: 5E6.1B4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free Summary

• Description: Novus Biologicals Mouse SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free (NBP2-36573) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-SLC31A1/CTR1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Two synthetic peptides made to internal sequences of human SLC31A1/CTR1 protein (sequences found between amino acids 50-150) [UniProt# O15431]
• Predicted Species: Rat (96%), Porcine (96%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC31A1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 2 ug/ml

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:32060280).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free

• Copper transporter 1
• COPT1
• COPT1high affinity copper uptake protein 1
• CTR1
• CTR1MGC75487
• HCTR1
• hCTR1copper transport 1 homolog
• SLC31A1
• solute carrier family 31 (copper transporters), member 1
• Solute carrier family 31 member 1

Background

SLC31A1/CTR1 (solute carrier family 31 member 1/copper transporter 1) is a homotrimeric high-affinity, saturable copper importer implicated in dietary copper uptake. Homeostasis of copper is tightly controlled by inter-regulatory circuitry containing copper, Sp1, and CTR1, wherein Sp1 transcription factor acts as a copper sensor in modulating CTR1 expression which in turn controls cellular copper and Sp1 levels in a 3-way mutual regulatory loop. CTR1 mediated copper uptake is time, temperature, and pH dependent and specific for reduced form of Cu (I) and copper reduction has been proposed to be caused by Steap protein family or Dcytb protein (Cybrd1) both of which are reported to possess cupric reductase activity. The intracellular localization of CTR1 is variable in different cell lines: CTR1 is localized at the plasma membrane in HEK293, CaCo-2, and A2780 cells, whereas, in other cells such as HeLa, it is predominantly localized to vesicular compartments, and variability in trafficking rates between vesicular and plasma membrane compartments has been suggested as the cause of this differential localization. Post-translational O-linked glycosylation of CTR1 protects it against proteolytic cleavage of N terminal 17-kD fragment and the biological function of this cleaved variant or protease responsible is largely unknown. Selective knockout of Ctr1 in murine intestinal epithelial cells results in severe systemic copper deficiency, ataxia, and death prior to weaning.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SLC31A1/CTR1 Antibody (NBP2-36573)(4)

Publications using NBP2-36573

• Bonet-Aleta J, Pezacki A, Oi M et al. Therapeutic Copper-based Nanoparticles Release Labile Copper(II) and Trigger Cellular Responses in Glutathione and NRF2 Redox Pathways and Metal Homeostasis ChemRxiv 2023-03-24 (WB)
• Ito F, Kato K, Yanatori I et al. Matrigel-based organoid culture of malignant mesothelioma reproduces cisplatin sensitivity through CTR1 BMC cancer 2023-05-31 [PMID: 37254056]
• Bonet-Aletá J, Pezacki A, Oi M et al. An Activity-Based Sensing Approach to Monitor Nanomaterial-Promoted Changes in Labile Metal Pools in Living Systems ChenRxiv 2023-06-15 (WB, Human)
  
  Details:
  Dilution 1:500
• Liao Y, Zhao J, Bulek K et al. Inflammation mobilizes copper metabolism to promote colon tumorigenesis via an IL-17-STEAP4-XIAP axis Nat Commun 2020-02-14 [PMID: 32060280] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: SLC31A1
• Entrez:
  • 1317(Human)
• Uniprot:
  • O15431(Human)