SLC31A1/CTR1 Antibody (3C7.1B7)

Western Blot: SLC31A1/CTR1 Antibody (3C7.1B7) [NBP2-36574] - Analysis of SLC31A1/CTR1 in MCF7 and HeLa at 2 ug/mL

Immunocytochemistry/Immunofluorescence: SLC31A1/CTR1 Antibody (3C7.1B7) [NBP2-36574] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti SLC31A1/CTR1 (3C7.1B7) at 1:50 overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at 1:500. Actin was detected with Phalloidin 568 (Red) at 1:200. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody (3C7.1B7) [NBP2-36574] - Analysis of a FFPE tissue section of normal skin from a human breast cancer patient (NBP2-30234) using purified CTR1/SLC31A1 antibody clone 3C7.1B7 at 5 ug/mL concentration. The antibody developed a strong cytoplasmic staining in the outer layer of stratum spinosum and the cells of stratum granulosum/ granular layer of skin epidermis.

Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody (3C7.1B7) [NBP2-36574] - Analysis of a FFPE tissue section of human skeletal muscle from a breast cancer patient (NBP2-30234) using purified CTR1/SLC31A1 antibody clone 3C7.1B7 at 5 ug/mL. The antibody generated moderate to strong cytoplasmic staining in the muscle cells/ myocytes.

Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody (3C7.1B7) [NBP2-36574] - Analysis of a FFPE tissue section of normal breast tissue from a human breast cancer patient (NBP2-30234) using purified CTR1/SLC31A1 antibody clone 3C7.1B7 at 5 ug/mL. The antibody generated strong cytoplasmic staining in the myo-epithelial cells in the tested section.

Product Discontinued

SLC31A1/CTR1 Antibody (3C7.1B7) Summary

• Immunogen: Two synthetic peptides made to internal sequences of human SLC31A1/CTR1 protein (sequences found between amino acids 50-150) [UniProt O15431]
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC31A1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Western Blot 2 ug/mL

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:32010131).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for SLC31A1/CTR1 Antibody (3C7.1B7)

• Copper transporter 1
• COPT1
• COPT1high affinity copper uptake protein 1
• CTR1
• CTR1MGC75487
• HCTR1
• hCTR1copper transport 1 homolog
• SLC31A1
• solute carrier family 31 (copper transporters), member 1
• Solute carrier family 31 member 1

Background

SLC31A1/CTR1 (solute carrier family 31 member 1/copper transporter 1) is a homotrimeric high-affinity, saturable copper importer implicated in dietary copper uptake. Homeostasis of copper is tightly controlled by inter-regulatory circuitry containing copper, Sp1, and CTR1, wherein Sp1 transcription factor acts as a copper sensor in modulating CTR1 expression which in turn controls cellular copper and Sp1 levels in a 3-way mutual regulatory loop. CTR1 mediated copper uptake is time, temperature, and pH dependent and specific for reduced form of Cu (I) and copper reduction has been proposed to be caused by Steap protein family or Dcytb protein (Cybrd1) both of which are reported to possess cupric reductase activity. The intracellular localization of CTR1 is variable in different cell lines: CTR1 is localized at the plasma membrane in HEK293, CaCo-2, and A2780 cells, whereas, in other cells such as HeLa, it is predominantly localized to vesicular compartments, and variability in trafficking rates between vesicular and plasma membrane compartments has been suggested as the cause of this differential localization. Post-translational O-linked glycosylation of CTR1 protects it against proteolytic cleavage of N terminal 17-kD fragment and the biological function of this cleaved variant or protease responsible is largely unknown. Selective knockout of Ctr1 in murine intestinal epithelial cells results in severe systemic copper deficiency, ataxia, and death prior to weaning.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SLC31A1/CTR1 Antibody (NBP2-36574)(1)

Publication using NBP2-36574

• Cichon I, Ortmann W, Bednarz A et al. Reduced Neutrophil Extracellular Trap (NET) Formation During Systemic Inflammation in Mice With Menkes Disease and Wilson Disease: Copper Requirement for NET Release Front. Immunol. 2020-01-15 [PMID: 32010131] (ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: SLC31A1
• Entrez:
  • 1317(Human)
• Uniprot:
  • O15431(Human)