SLC22A12 Overexpression Lysate

Images

 
Western Blot: SLC22A12 Overexpression Lysate (Adult Normal) [NBL1-16031] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SLC22A12.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SLC22A12 Overexpression Lysate Summary

Description

SLC22A12 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC217294

Accession#: NM_144585

Protein Tag: C-MYC/DDK
Gene
SLC22A12

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
59.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SLC22A12 Overexpression Lysate

  • OATL4
  • Organic anion transporter 4-like protein
  • Renal-specific transporter
  • RST
  • RSTmember 12
  • SLC22A12
  • solute carrier family 22 (organic anion/urate transporter), member 12
  • UNQ6453/PRO34004
  • URAT1
  • URAT1Urate anion exchanger 1
  • urate transporter 1

Background

The protein encoded by this gene is a urate transporter and urate-anion exchanger which regulates the level of urate in the blood. This protein is an integral membrane protein primarily found in kidney. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for SLC22A12 Lysate (NBL1-16031) (0)

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Video Protocols

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Bioinformatics

Gene Symbol SLC22A12