SLAM/CD150 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

SLAM/CD150 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human SLAM/CD150
  Ala21-Pro237
  Accession # Q13291
• Specificity: Detects human SLAM/CD150 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse SLAM, recombinant human (rh) CD48, rhCD84, and rh2B4/CD244 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SLAMF1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SLAM/CD150 Antibody [Biotin]

• CD 150
• CD150
• CD150IPO-3
• CDw150
• IPO-3
• signaling lymphocytic activation molecule family member 1
• signaling lymphocytic activation molecule
• SLAM
• SLAMCD150 antigen
• SLAMF1

Background

SLAM (also IPO-3 and CD150) is a 70‑80 kDa member of the CD2 family of molecules. It is a costimulatory receptor that mediates T cell activation via SAP. SLAM is found on B cells, dendritic cells, macrophages, stem cells and Th1 T cells. SLAM engages in homophilic interactions. Mature human SLAM is 315 amino acids (aa) in length. It is a type I transmembrane glycoprotein whose extracellular domain contains one V-type and one C2-type Ig-like domain (aa 21‑237). There is a 77 aa SH2‑binding cytoplasmic domain. Four potential splice variants are suggested. One shows a deletion of aa 234‑263 (potentially soluble), while three others show aa substitutions; i.e.‑a ten aa substitution for aa 289‑335, a six aa substitution for aa 264‑335 and a 20 aa substitution for aa 125‑335 (potentially soluble). Over aa 1‑236, human SLAM shares 60% aa identity with mouse SLAM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLAMF1
• Uniprot:
  • Q13291()