SLAM/CD150 Antibody [Alexa Fluor® 750]

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 750

SLAM/CD150 Antibody [Alexa Fluor® 750] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse SLAM/CD150
  Thr25-Pro242
  Accession # Q9QUM4
• Specificity: Detects mouse SLAM/CD150 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human SLAM is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SLAM/CD150 Antibody [Alexa Fluor® 750]

• CD 150
• CD150
• CD150IPO-3
• CDw150
• IPO-3
• signaling lymphocytic activation molecule family member 1
• signaling lymphocytic activation molecule
• SLAM
• SLAMCD150 antigen
• SLAMF1

Background

The type I transmembrane glycoprotein Signaling Lymphocytic Activation Molecule (SLAM), also known as CD150, is the prototypic member of the SLAM subgroup of the CD2 protein family. CD2 family proteins function as adhesion molecules and modulators of the immune response (1). Mouse SLAM consists of a 218 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 23 aa transmembrane segment, and a 78 aa cytoplasmic domain with three immunoreceptor tyrosine switch motifs (ITSM) (2). Alternate splicing generates an isoform with a substituted cytoplasmic domain (2). Within the ECD, mouse SLAM shares 58% and 83% aa sequence identity with human and rat SLAM, respectively. It is expressed as a 75 kDa molecule of which approximately 30 kDa is N-linked carbohydrate (2). SLAM is expressed on T cells, B cells, thymocytes, macrophages, dendritic cells, platelets, and hematopoietic stem cells (2‑7). It is upregulated on activated B cells and CD4⁺ and CD8⁺ T cells, although it is downregulated on Th2 polarized cells (2, 3, 8). SLAM interacts homophilically with low affinity, and this interaction induces a Th0/Th1 response characterized by clonal expansion, production of IFN-gamma , and increased cytolytic activity of CD8⁺ T cells (2, 3, 9‑11). SLAM ligation also promotes B cell activation, allergen-induced eosinophil and mast cell activation, and macrophage responsiveness to LPS (4, 8, 12). In humans, SLAM functions as a cellular entry receptor for measles virus (13, 14).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.