SKT Antibody

Immunocytochemistry/Immunofluorescence: SKT Antibody [NBP1-84100] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus & cytosol.

Immunohistochemistry-Paraffin: SKT Antibody [NBP1-84100] - Staining of human colon shows moderate cytoplasmic and membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: SKT Antibody [NBP1-84100] - Staining of human testis shows moderate cytoplasmic and membranous positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: SKT Antibody [NBP1-84100] - Staining of human Fallopian tube shows moderate membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: SKT Antibody [NBP1-84100] - Staining of human kidney shows moderate cytoplasmic and membranous positivity in cells in distal tubules.

• Reactivity: Hu
• Applications: ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

SKT Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: KEEPHKLDSLLKRVRSMTDVLTMLRRHVTDGLLKGTDAAQAAQYMAMEKATAAEVLKSQEEAAHTSGQPFHSTGAPGDAKSEVVPLSGMMVRHAQSSPVVIQPSQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KIAA1217
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  SKT Protein (NBP1-84100PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (86%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SKT Antibody

• DKFZP761L0424
• KIAA1217
• likely orthologue of Mus musculus enhancer trap locus 4
• MGC31990
• sickle tail protein homolog
• SKT

Background

SKT is required for normal development of intervertebral disks.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KIAA1217