SIT1 Antibody [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 405

SIT1 Antibody [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human SIT1
  Gln65-Ser196
  Accession # Q9Y3P8
• Specificity: Detects human SIT1 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SIT1 Antibody [Alexa Fluor® 405]

• gp30/40
• MGC125908
• MGC125909
• RP11-331F9.5
• SHP2 interacting transmembrane adaptor
• SHP2-interacting transmembrane adapter protein
• SHP2-interacting transmembrane adaptor protein
• signaling threshold regulating transmembrane adaptor 1
• signaling threshold-regulating transmembrane adapter 1
• SIT1
• SITMGC125910
• suppression inducing transmembrane adaptor 1
• Suppression-inducing transmembrane adapter 1

Background

SIT1 (SHP-2-interacting transmembrane adapter 1; also signaling threshold-regulating transmembrane adapter 1) is a 35‑40 kDa type I transmembrane glycoprotein that belongs to a diverse group of transmembrane adapter proteins. It is expressed on thymocytes, plasma cells and T cells. When engaged, SIT1 downregulates TCR signaling. Mature human SIT1 is 172 amino acids (aa) in length. It contains a short extracellular domain (aa 25‑40) that undergoes homodimerization and an extensive intracellular region (aa 62‑196) that contains three key phosphorylation sites (Tyr90/169/188). Over aa 65‑196, human SIT1 is 86% and 80% aa identical to canine and mouse SIT1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.