Sirtuin 2/SIRT2 Antibody - BSA Free

Western Blot: Sirtuin 2/SIRT2 Antibody [NBP1-76880] - Human brain lysate with SIRT2 antibody at (A) 2.5 and (B) 5 ug/mL.

Immunohistochemistry-Paraffin: Sirtuin 2/SIRT2 Antibody [NBP1-76880] - Human brain tissue using Sirt2 antibody at 2.5 ug/ml.

Immunocytochemistry/ Immunofluorescence: Sirtuin 2/SIRT2 Antibody - BSA Free [NBP1-76880] - Immunofluorescence of sirt2 in human brain tissue with sirt2 antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Sirtuin 2/SIRT2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Sirtuin 2/SIRT2 Antibody - BSA Free (NBP1-76880) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of the human SIRT2. The immunogen is located within the first 50 amino acids of SIRT2. Amino Acid Squence: AGKVQEAQDSDSDSEGG
• Marker: Early Oligodendrocyte Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SIRT2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 2.5-5 ug/ml
• Control Peptide:

  Sirtuin 2/SIRT2 Peptide (NBP1-76880PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Sirtuin 2/SIRT2 Antibody - BSA Free

• EC 3.5.1
• EC 3.5.1.-
• FLJ35621
• FLJ37491
• S.cerevisiae, homolog) 2
• SIR2L2
• SIRT2
• Sirtuin 2
• sirtuin type 2

Background

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG16, another member of the autophagy protein family, forms a complex with the ATG5-ATG12 conjugate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-terminal domain, it has been suggested that these may form a platform for further protein-protein interactions. Multiple isoforms of ATG16 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIRT2
• Uniprot:
  • NP_036369(Human)