SIL1 Antibody (OTI3E3) - Azide and BSA Free

Images

 
Western Blot: SIL1 Antibody (OTI3E3) - Azide and BSA Free [NBP2-74193] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: SIL1 Antibody (OTI3E3) - Azide and BSA Free [NBP2-74193] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SIL1.
Immunohistochemistry: SIL1 Antibody (OTI3E3) - Azide and BSA Free [NBP2-74193] - Staining of paraffin-embedded Kidney tissue using anti-SIL1 mouse monoclonal antibody.
Flow Cytometry: SIL1 Antibody (OTI3E3) - Azide and BSA Free [NBP2-74193] - HEK293T cells transfected with either pCMV6-ENTRY SIL1.(Red) or empty vector control plasmid (Blue) were immunostaining with anti-SIL1 mouse ...read more
Immunocytochemistry/ Immunofluorescence: SIL1 Antibody (OTI3E3) - Azide and BSA Free [NBP2-74193] - Staining of HT29 cells using anti-SIL1 mouse monoclonal antibody.
Immunohistochemistry: SIL1 Antibody (OTI3E3) - Azide and BSA Free [NBP2-74193] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-SIL1 mouse monoclonal antibody.
Immunohistochemistry: SIL1 Antibody (OTI3E3) - Azide and BSA Free [NBP2-74193] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-SIL1 mouse monoclonal antibody.
Immunohistochemistry: SIL1 Antibody (OTI3E3) - Azide and BSA Free [NBP2-74193] - Staining of paraffin-embedded liver tissue using anti-SIL1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI3E3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

SIL1 Antibody (OTI3E3) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human SIL1 (NP_071909) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SIL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100-500
  • Western Blot 1:200
Theoretical MW
52.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for SIL1 Antibody (OTI3E3) - Azide and BSA Free

  • BAPMarinesco-Sjogren syndrome
  • BiP-associated protein
  • MSS
  • nucleotide exchange factor SIL1
  • SIL1 homolog, endoplasmic reticulum chaperone (S. cerevisiae)
  • ULG5

Background

SIL1 is a resident endoplasmic reticulum (ER) glycoprotein that interacts with the ATPase domain of BIP and enhances nucleotide exchange.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SIL1