Siglec-6/CD327 Antibody [Unconjugated]

Recombinant Human Siglec‑6/CD327 protein was serially diluted 2-fold and captured by Mouse Anti-Human Siglec‑6/CD327 Monoclonal Antibody (Catalog # MAB28591) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Siglec‑6/CD327 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2859) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Siglec-6/CD327 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Siglec‑6/CD327
  Gln27-Val331
  Accession # NP_942142
• Specificity: Detects human Siglec‑6/CD327 in direct ELISAs and Western blots. In Western blots, approximately 20% cross‑reactivity with recombinant human (rh) Siglec-3, rhSiglec-5 and rhSiglec-9 is observed, approximately 5% cross‑reactivity with rhSiglec-10 is observed, and less than 1% cross‑reactivity with rhSiglec-2, rhSiglec-7, and rhSiglec-11 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SIGLEC6
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Siglec-6/CD327 Antibody [Unconjugated]

• CD327 antigen
• CD327
• CD33 antigen-like 1
• CD33L1
• CD33L1OBBP1CD327
• CD33L2
• CD33LCDW327
• CDw327
• OB-BP1
• Obesity-binding protein 1
• sialic acid binding Ig-like lectin 6
• sialic acid-binding Ig-like lectin 6
• Siglec6
• Siglec-6

Background

Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins that belong to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1‑4). Eleven human Siglecs (Siglec-1 through 11) have been cloned and characterized. Within these eleven, there are at least two groups, one of which is termed the CD33-related group. CD33-related Siglecs include CD33/Siglec-3 and Siglec-5 through 11 (1, 3). To date, no Siglec has been shown to recognize any cell surface ligand other than sialic acid. This suggests that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. The cDNA of human Siglec-6 (also known as OB-BP1 and CD33L), encodes a putative 442 amino acid (aa) protein that contains a 15 aa signal peptide, a 321 aa extracellular region, a 21 aa transmembrane region (TM), and an 85 aa cytoplasmic tail (5, 6). The extracellular region contains one N-terminal V-type Ig-like domain followed by two Ig-like C2-type domains. The cytoplasmic domain has one immunoreceptor tyrosine-based inhibition motif (ITIM). At least three additional isoforms exist, all of which encode an additional 11 aa’s at the N-terminus, likely due to the utilization of an alternate start site. Two of the three isoforms also show splicing. One isoform shows a 16 aa in-frame deletion in the second C2-like domain, while the other shows a deletion of the TM and cytoplasmic region, thus potentially generating a soluble form (6‑9). Siglec-6 is found on B cells and in placenta, and would seem to have a restricted specificity for the sialyl Tn antigen (6, 10).

1. Crocker, P.R. and J. Zhang (2002) Biochem. Soc. Symp. 69:83.
2. Crocker, P.R. and A. Varki (2001) Immunology 103:137.
3. Crocker, P.R. (2002) Curr. Opin. Struct. Biol. 12:609.
4. Powell, L.D. and A. Varki (1995) J. Biol. Chem. 270:14243.
5. Takei, Y. et al. (1997) Cytogenet. Cell Genet. 78:295.
6. Patel, N. et al. (1999) J. Biol. Chem. 274:22729.
7. GenBank Accession # NP_942143.
8. GenBank Accession # NP_942142.
9. GenBank Accession # NP_001236.
10. Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Siglec-6/CD327 Antibody (AF2859)(2)

Publications using AF2859

• AL Stefanski, MD Renecle, A Kramer, S Sehgal, P Narasimhan, KK Rumer, VD Winn Siglec-6 Signaling Uses Src Kinase Tyrosine Phosphorylation and SHP-2 Recruitment Cells, 2022-10-29;11(21):. 2022-10-29 [PMID: 36359823] (Western Blot, Human)
• Lam KK, Chiu PC, Lee CL Glycodelin-A protein interacts with Siglec-6 protein to suppress trophoblast invasiveness by down-regulating extracellular signal-regulated kinase (ERK)/c-Jun signaling pathway. J. Biol. Chem., 2011-08-31;286(43):37118-27. 2011-08-31 [PMID: 21880722] (ICC, Western Blot, Human)

Bioinformatics

• Gene Symbol: SIGLEC6
• Uniprot:
  • NP_942142()