Siglec-6/CD327 Antibody [CoraFluor™ 1]

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: CoraFluor 1

Siglec-6/CD327 Antibody [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Siglec‑6/CD327
  Gln27-Val331
  Accession # NP_942142
• Specificity: Detects human Siglec‑6/CD327 in direct ELISAs and Western blots. In Western blots, approximately 20% cross‑reactivity with recombinant human (rh) Siglec-3, rhSiglec-5 and rhSiglec-9 is observed, approximately 5% cross‑reactivity with rhSiglec-10 is observed, and less than 1% cross‑reactivity with rhSiglec-2, rhSiglec-7, and rhSiglec-11 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SIGLEC6
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Siglec-6/CD327 Antibody [CoraFluor™ 1]

• CD327 antigen
• CD327
• CD33 antigen-like 1
• CD33L1
• CD33L1OBBP1CD327
• CD33L2
• CD33LCDW327
• CDw327
• OB-BP1
• Obesity-binding protein 1
• sialic acid binding Ig-like lectin 6
• sialic acid-binding Ig-like lectin 6
• Siglec6
• Siglec-6

Background

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds to alpha-2,6-linkedsialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cellsurface

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIGLEC6