Pappalysin-2/PAPP-A2 Antibody

Effects of gene disruption on PAPP-A2 protein.Shown are Western blots of 9 representative (A) placentae and (B) embryos at embryonic day 12.5 from embryos homozygous for wild-type Pappa2 (+/+), heterozygous (+/−) or homozygous for the disruption (−/−). The nitrocellulose membrane was scanned for fluorescence at 700 and 800 nm simultaneously, with fluorescence at 700 nm corresponding to actin (at approximately 40 kDa) and fluorescence at 800 nm corresponding to PAPP-A2 (at approximately 250 kDa). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23457539), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Pappalysin-2/PAPP-A2 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Pappalysin‑2/PAPP‑A2
  Ser234-Cys1396
  Accession # Q9BXP8
• Specificity: Detects human Pappalysin‑2/PAPP‑A2 in direct ELISAs and Western blots. In Western blots, less than 1% cross‑reactivity with recombinant human PAPP-A is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PAPPA2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pappalysin-2/PAPP-A2 Antibody

• EC 3.4.24.-
• PAPPA2
• PAPP-A2
• PAPP-A2pappalysin-2
• pappalysin 2
• Pappalysin2
• Pappalysin-2
• PAPPE
• PAPP-E
• PLAC3
• placenta-specific 3
• pregnancy-associated plasma preproprotein-A2
• Pregnancy-associated plasma protein A2
• Pregnancy-associated plasma protein E1

Background

Pappalysins belong to a fifth family of metzincins that consists of ADAMs/ADAMTSs, MMPs, astacins and serrylysins (1, 2). PAPP-A is an important pregnancy protein and increases in plasma by a factor of about 150 during pregnancy as compared to the nonpregnant state. PAPP-A is also a major marker of Down syndrome in the first trimester of pregnancy because maternal serum levels of PAPP-A are significantly reduced when a fetus affected by Down syndrome is present (3). PAPP-A cleaves Insulin-like Growth Factor-Binding Protein-4 and -5 (IGFBP-4 and -5) at a single site, resulting in the release of bioactive IGF (4). Compared to PAPP‑A, PAPP-A2 (also called PAPP-E), the second member of the family is less characterized. PAPP-A2 shares 45% amino acid identity to PAPP-A in the mature form, which is synthesized as a preproprotein consisting of multiple domains (1). The prepro region (residues 1‑233) and the C-terminal region (residues 1397‑1791) are not included in recombinant human PAPP-A2. As an active protease, recombinant human PAPP-A2 cleaves IGFBP-5.

1. Overgaard, M.T. et al. (2001) J. Biol. Chem. 276:21849.
2. Boldt, H.B. et al. (2001) Biochem. J. 358:359.
3. Fialova L. and I.M. Malbohan (2002) Bratisl. Lek. Listy 103:194.
4. Laursen, L.S. et al. (2001) FEBS Lett. 504:36.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pappalysin-2/PAPP-A2 Antibody (AF1668)(8)

Publications using AF1668

• Katherine Xu, Paul Rosenstiel, Neal Paragas, Christian Hinze, Xiaobo Gao, Tian Huai Shen et al. Unique Transcriptional Programs Identify Subtypes of AKI Journal of the American Society of Nephrology 2017-06-01 [PMID: 28028135]
• Crosley , Erin J, Dunk , Caroline, Beristain , Alexande, Christians , Julian K IGFBP-4 and -5 are expressed in first-trimester villi and differentially regulate the migration of HTR-8/SVneo cells. Reprod Biol Endocrinol, 2014-12-04;12(0):123. 2014-12-04 [PMID: 25475528] (IHC-P, Human)
• Erin J. Crosley, Ursula Durland, Ken Seethram, Scott MacRae, Andrée Gruslin, Julian K. Christians First-Trimester Levels of Pregnancy-Associated Plasma Protein A2 (PAPP-A2) in the Maternal Circulation Are Elevated in Pregnancies That Subsequently Develop Preeclampsia Reproductive Sciences 2014-06-01 [PMID: 24336677]
• Julian Kenneth Christians, Devin Rhys de Zwaan, Sunny Ho Yeung Fung Pregnancy Associated Plasma Protein A2 (PAPP-A2) Affects Bone Size and Shape and Contributes to Natural Variation in Postnatal Growth in Mice PLoS ONE 2013-02-15 [PMID: 23457539]
• Pamela K Wagner, Aki Otomo, Julian K Christians Regulation of pregnancy-associated plasma protein A2 (PAPPA2) in a human placental trophoblast cell line (BeWo) Reproductive Biology and Endocrinology 2011-12-01 [PMID: 21496272]
• Pamela K Wagner, Julian K Christians Altered placental expression of PAPPA2 does not affect birth weight in mice Reproductive Biology and Endocrinology 2010-07-20 [PMID: 20642865] (Western Blot, Mouse)
• Wang J, Qiu Q, Haider M, Bell M, Gruslin A, Christians JK Expression of pregnancy-associated plasma protein A2 during pregnancy in human and mouse. J. Endocrinol., 2009-05-27;202(3):337-45. 2009-05-27 [PMID: 19474058] (IHC-P, Western Blot, Human, Mouse)
• Nishizawa H, Pryor-Koishi K, Suzuki M Increased levels of pregnancy-associated plasma protein-A2 in the serum of pre-eclamptic patients. Mol. Hum. Reprod., 2008-09-18;14(10):595-602. 2008-09-18 [PMID: 18805800] (ELISA Development, IHC-P, Western Blot, Human)

Review for Pappalysin-2/PAPP-A2 Antibody (AF1668) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 01/05/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 23457539
• Species: Mouse

Bioinformatics

• Gene Symbol: PAPPA2
• Uniprot:
  • Q9BXP8()