Siglec-3/CD33 Antibody (WM53) - Low Endotoxin

Flow Cytometry: Siglec-3/CD33 Antibody (WM53) - Low Endotoxin, Azide and BSA Free [NBP3-09095] - Mass cytometry (surface staining) of human peripheral blood with anti-CD33 (WM53) 149Sm. Gated on singlets.

Flow Cytometry: Siglec-3/CD33 Antibody (WM53) - Low Endotoxin, Azide and BSA Free [NBP3-09095] - Analysis (surface staining) of human peripheral blood with anti-CD33 (WM53) purified antibody (low endotoxin), GAM-APC.

• Reactivity: Hu, Pm
• Applications: WB, Flow, Func, ICC/IF, IHC, IP, CyTOF-ready
• Clone: WM53
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Low Endotoxin
• Concentration: 1 mg/ml

Siglec-3/CD33 Antibody (WM53) - Low Endotoxin Summary

• Additional Information: Clone WM53 was used by HLDA to establish CD designation.
• Immunogen: Human AML cells
• Specificity: The mouse monoclonal antibody WM53 reacts with an extracellular epitope of CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD33
• Purity: Protein A purified
• Endotoxin Note: Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1-4 ug/ml
  • Functional
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 0.2 um filter sterilized
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Siglec-3/CD33 Antibody (WM53) - Low Endotoxin

• CD33 antigen (gp67)
• CD33 antigen
• CD33 molecule
• CD33
• FLJ00391
• gp67
• myeloid cell surface antigen CD33
• p67
• sialic acid binding Ig-like lectin 3
• Sialic acid-binding Ig-like lectin 3
• Siglec3
• Siglec-3
• SIGLEC3gp67

Background

CD33 is a 67 kDa type I transmembrane glycoprotein and a member of the sialoadhesin family of cell surface receptors. It is absent from pluripotent stem cells but appears on myelomonocytic precursors after CD34. It then continues to be expressed on both the myeloid and monocyte lineages, although it is absent on granulocytes. While it has been reported that CD33 can function as a sialic acid-dependent cell adhesion molecule, cells expressing CD33 require desialylation before they can bind cells bearing the appropriate sialoglycoconjugates. This suggests that inhibitory cis interactions may regulate or block any adhesion function. (1-4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD33