Siglec-3/CD33 Antibody (WM53) - Azide and BSA Free

Flow Cytometry: Siglec-3/CD33 Antibody (WM53) - Azide and BSA Free [NBP2-34511] - Monocyte differentiation stages characterized by CD45, CD34, CD33 (NBP2-34511PECY7) and CD14 expression. HLA-DR and CCR2 expression were measured in each stage and expressed as the number of sites per cell. A) example of FACs analysis for monocyte differentiation (red: myelo/monoblasts, blue: promonocytes, green: monocytes), and the HLA-DR and CCR2 expression levels in each stage. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27893741/) licensed under a CC-BY license.

• Reactivity: Hu
• Applications: Flow, ICC/IF, CyTOF-ready
• Clone: WM53
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

Siglec-3/CD33 Antibody (WM53) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32931).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Human AML cells
• Localization: Cell surface
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD33
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/ Immunofluorescence 0.5-1ug/ml
• Application Notes: Optimal dilution for a specific application should be determined.
• Theoretical MW: 67 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for Siglec-3/CD33 Antibody (WM53) - Azide and BSA Free

• CD33 antigen (gp67)
• CD33 antigen
• CD33 molecule
• CD33
• FLJ00391
• gp67
• myeloid cell surface antigen CD33
• p67
• sialic acid binding Ig-like lectin 3
• Sialic acid-binding Ig-like lectin 3
• Siglec3
• Siglec-3
• SIGLEC3gp67

Background

CD33 is a 67 kDa type I transmembrane glycoprotein and a member of the sialoadhesin family of cell surface receptors. It is absent from pluripotent stem cells but appears on myelomonocytic precursors after CD34. It then continues to be expressed on both the myeloid and monocyte lineages, although it is absent on granulocytes. While it has been reported that CD33 can function as a sialic acid-dependent cell adhesion molecule, cells expressing CD33 require desialylation before they can bind cells bearing the appropriate sialoglycoconjugates. This suggests that inhibitory cis interactions may regulate or block any adhesion function. (1-4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD33