Siglec-3/CD33 Antibody (6C5/2) - BSA Free

• Reactivity: Hu
• Applications: ELISA, Flow, IHC, CyTOF-ready
• Clone: 6C5/2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Siglec-3/CD33 Antibody (6C5/2) - BSA Free Summary

• Description: Novus Biologicals Mouse Siglec-3/CD33 Antibody (6C5/2) - BSA Free (NBP2-50292) is a monoclonal antibody validated for use in IHC, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Siglec-3/CD33-Fc recombinant protein
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD33
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunohistochemistry 1:10 - 1:500
• Application Notes: Positive control(s): CD33 transfected COS cells. This antibody is Cytof ready.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Siglec-3/CD33 Antibody (6C5/2) - BSA Free

• CD33 antigen (gp67)
• CD33 antigen
• CD33 molecule
• CD33
• FLJ00391
• gp67
• myeloid cell surface antigen CD33
• p67
• sialic acid binding Ig-like lectin 3
• Sialic acid-binding Ig-like lectin 3
• Siglec3
• Siglec-3
• SIGLEC3gp67

Background

CD33 is a 67 kDa type I transmembrane glycoprotein and a member of the sialoadhesin family of cell surface receptors. It is absent from pluripotent stem cells but appears on myelomonocytic precursors after CD34. It then continues to be expressed on both the myeloid and monocyte lineages, although it is absent on granulocytes. While it has been reported that CD33 can function as a sialic acid-dependent cell adhesion molecule, cells expressing CD33 require desialylation before they can bind cells bearing the appropriate sialoglycoconjugates. This suggests that inhibitory cis interactions may regulate or block any adhesion function. (1-4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD33