Siglec-2/CD22 Antibody (2H1C4)

Images

 
Western Blot: Siglec-2/CD22 Antibody (2H1C4) [NBP2-37327] - Analysis using CD22 mouse mAb against L1210 (1), Hela (2), HEK293 (3), Jurkat (4), OCM-1 (5), A432 (6) and NIH/3T3 (7) cell lysate.
Immunocytochemistry/ Immunofluorescence: Siglec-2/CD22 Antibody (2H1C4) [NBP2-37327] - Immunofluorescence analysis of Hela cells using CD22 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have ...read more
Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (2H1C4) [NBP2-37327] - Analysis of cerebellum tissues using CD22 mouse mAb with DAB staining.
Flow Cytometry: Siglec-2/CD22 Antibody (2H1C4) [NBP2-37327] - Analysis of Hela cells using CD22 mouse mAb (green) and negative control (red).
Western Blot: Siglec-2/CD22 Antibody (2H1C4) [NBP2-37327] - Analysis using CD22 mAb against human CD22 recombinant protein. (Expected MW is 37 kDa).
Western Blot: Siglec-2/CD22 Antibody (2H1C4) [NBP2-37327] - Analysis using CD22 mAb against HEK293 (1) and CD22 (AA: 621-725)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (2H1C4) [NBP2-37327] - Analysis of cervical cancer tissues using CD22 mouse mAb with DAB staining.
ELISA: Siglec-2/CD22 Antibody (2H1C4) [NBP2-37327] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
2H1C4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Siglec-2/CD22 Antibody (2H1C4) Summary

Immunogen
Purified recombinant fragment of human CD22 (AA: 621-725) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD22
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100- 1:500
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin
Theoretical MW
95.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for Siglec-2/CD22 Antibody (2H1C4)

  • B-cell receptor CD22
  • BL-CAM
  • B-lymphocyte cell adhesion molecule
  • CD22 antigenMGC130020
  • CD22 molecule
  • CD22
  • sialic acid binding Ig-like lectin 2
  • Sialic acid-binding Ig-like lectin 2
  • Siglec2
  • Siglec-2
  • SIGLEC2FLJ22814
  • T-cell surface antigen Leu-14

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Siglec-2/CD22 Antibody (NBP2-37327). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in buying a CD22 antibody for fluorescence microscopy and FACS I red on your web page that you also offer to label your antibodies, if required, is that correct?
    • We do have the ability to perform custom conjugations on certain antibodies, including some to the CD22 protein. If you send me the conjugate you are interested in I can provide you with a quote.

Secondary Antibodies

 

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Blogs on Siglec-2/CD22.

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Bioinformatics

Gene Symbol CD22