Siglec-15 Antibody (1026113) [Alexa Fluor™ Plus 405]

• Reactivity: Hu
• Clone: 1026113
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 405

Siglec-15 Antibody (1026113) [Alexa Fluor™ Plus 405] Summary

• Specificity: Detects human Siglec-15 in direct ELISAs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Siglec-15 Antibody (1026113) [Alexa Fluor™ Plus 405]

• CD33 antigen-like 3SIGLEC-15
• CD33 molecule-like 3
• CD33L3
• HsT1361
• sialic acid binding Ig-like lectin 15
• sialic acid-binding Ig-like lectin 15
• Siglec15
• Siglec-15

Background

Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules (1). Mature human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain (2). Within the ECD, human Siglec-15 shares 85% aa sequence identity with mouse and rat Siglec-15. Alternative splicing generates an additional isoform that lacks the signal peptide and first Ig-like domain. Siglec-15 associates with the signaling molecules DAP12 and DAP10 (2-5). It is expressed on osteoclasts, macrophages, and dendritic cells (2-6) and binds to the sialyl-Tn antigen (2, 3, 6). This interaction induces the production of TGF-beta by tumor-associated macrophages (3). Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts (4-6).

1. Humphrey, M.B. and M.C. Nakamura (2015) Clin. Rev. Allergy Immunol. PMID 26573914.
2. Angata, T. et al. (2007) Glycobiology 17:838.
3. Takamiya, R. et al. (2013) Glycobiology 23:178.
4. Kameda, Y. et al. (2013) J. Bone Miner. Res. 28:2463.
5. Stuible, M. et al. (2014) J. Biol. Chem. 289:6498.
6. Ishida-Kitagawa, N. et al. (2012) J. Biol. Chem. 287:17493.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.