Siglec-10 Antibody [Biotin]

• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Siglec-10 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Siglec-10
  Met17-Thr546
  Accession # Q96LC7
• Specificity: Detects human Siglec-10 in Western blots. In Western blots, less than 2% cross-reactivity with recombiant human (rh) Siglec-2, rhSiglec-3, rhSiglec-5, rhSiglec-7, rhSiglec-9, and recombinant mouse Siglec-F is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SIGLEC10
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N‑terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5‑11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system (1‑3). Siglec-G is the apparent ortholog of human Siglec-10 (4). The human Siglec-10 cDNA encodes a 697 amino acid (aa) precursor that includes a 16 aa signal sequence, a 534 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 126 aa cytoplasmic domain. The ECD contains one Ig-like V‑type domain and four Ig-like C2-type domains, while the cytoplasmic domain contains two immunoreceptor tyrosine-based inhibitory motifs (ITIM) (5‑8). Five splice variants of human Siglec-10 differ in their deletions within the ECD. A potentially secreted sixth variant contains the Ig-like V-type domain followed by a 45 aa substitution (5‑7, 9). Within the ECD, human Siglec-10 is most closely related to Siglec-5 (42% aa sequence identity). It shares 63% aa sequence identity with mouse Siglec-G. Siglec-10 is expressed on eosinophils, neutrophils, monocytes, and B cells (5, 8) with some splice variants predominating in particular cell types and tissue locations (6, 7, 9). It is up‑regulated on eosinophils in mouse models of allergic respiratory inflammation (10). Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides (7, 11). This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell (7). When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals (5, 9).

1. Crocker, P.R. (2005) Curr. Opin. Pharmacol. 5:431.
2. Crocker, P.R. (2002) Curr. Opin. Struct. Biol. 12:609.
3. Crocker, P.R. and J. Zhang (2002) Biochem. Soc. Symp. 69:83.
4. Angata, T. et al. (2001) J. Biol. Chem. 276:45128.
5. Whitney, G. et al. (2001) Eur. J. Biochem. 268:6083.
6. Yousef, G.M. et al. (2001) Biochem. Biophys. Res. Commun. 284:900.
7. Li, N. et al. (2001) J. Biol. Chem. 276:28106.
8. Munday, J. et al. (2001) Biochem. J. 355:489.
9. Kitzig, F. et al. (2002) Biochem. Biophys. Res. Commun. 296:355.
10. Aizawa, H. et al. (2003) Genomics 82:521.
11. Rapoport, E. et al. (2003) Bioorg. Med. Chem. Lett. 13:675.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIGLEC10