Western Blot: SIAH1 Antibody [NB300-974] - (1ug/ml) of Human Liver lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot: SIAH1 Antibody [NB300-974] - (1ug/ml) staining of Mouse (A, C) and Rat (B, D) Brain (A, B) and Liver (C, D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by ...read more
IP successful usage from CSM14.1 cells is reported in scientific literature (Liman et al, Molecules 2010, 15, 6678-6687). WB: Approx. 28 kDa band observed in human liver lysates and in mouse and rat brain and liver lysates (calculated MW of 31.1 kDa band according to NP_003022.3).
31 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Predicted cross-reactivity based on sequence identity: Canine, Bovine.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for SIAH1 Antibody
E3 ubiquitin-protein ligase SIAH1
seven in absentia homolog 1 (Drosophila)
Seven in absentia homolog 1
This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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