SIAH1 Antibody

Images

 
Western Blot: SIAH1 Antibody [NB300-974] - (1ug/ml) of Human Liver lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot: SIAH1 Antibody [NB300-974] - (1ug/ml) staining of Mouse (A, C) and Rat (B, D) Brain (A, B) and Liver (C, D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IP, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

SIAH1 Antibody Summary

Immunogen
Peptide with sequence SRQTATALPTGTSKC corresponding to N-Terminus according to NP_003022.3, NP_001006611.1.
Specificity
This product is expected to recognize both reported isoforms (according to NP_003022.3; NP_001006611.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SIAH1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Immunoprecipitation 1:50
  • Peptide ELISA Detection limit 1:16000
Application Notes
IP successful usage from CSM14.1 cells is reported in scientific literature (Liman et al, Molecules 2010, 15, 6678-6687).
WB: Approx. 28 kDa band observed in human liver lysates and in mouse and rat brain and liver lysates (calculated MW of 31.1 kDa band according to NP_003022.3).
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NB300-974.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SIAH1 Antibody

  • E3 ubiquitin-protein ligase SIAH1
  • EC 6.3.2
  • EC 6.3.2.-
  • FLJ08065
  • hSIAH1
  • HUMSIAH
  • seven in absentia homolog 1 (Drosophila)
  • Seven in absentia homolog 1
  • Siah-1
  • SIAH1A
  • siah-1a

Background

This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SIAH1
Entrez
Uniprot