SIAH1 Antibody

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Immunocytochemistry/ Immunofluorescence: SIAH1 Antibody [NB300-974] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications ELISA, ICC/IF, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

SIAH1 Antibody Summary

Immunogen
Peptide with sequence SRQTATALPTGTSKC corresponding to N-Terminus according to NP_003022.3, NP_001006611.1.
Specificity
This product is expected to recognize both reported isoforms (according to NP_003022.3; NP_001006611.1).
Predicted Species
Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SIAH1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunoprecipitation
  • Peptide ELISA Detection limit 1:128000
Application Notes
Use in immunoprecipitation reported in scientific literature (PMID: 19479934).
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NB300-974.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SIAH1 Antibody

  • E3 ubiquitin-protein ligase SIAH1
  • EC 6.3.2
  • EC 6.3.2.-
  • FLJ08065
  • hSIAH1
  • HUMSIAH
  • seven in absentia homolog 1 (Drosophila)
  • Seven in absentia homolog 1
  • Siah-1
  • SIAH1A
  • siah-1a

Background

This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for SIAH1 Antibody (NB300-974) (0)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SIAH1
Entrez
Uniprot