SHP-2/PTPN11 Antibody

Western Blot: SHP-2/PTPN11 Antibody [NB100-806] - Human Muscle lysate (RIPA buffer, 35 ug total protein per lane). Antibody at 2 ug/mL. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: SHP-2/PTPN11 Antibody [NB100-806] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Flow Cytometry: SHP-2/PTPN11 Antibody [NB100-806] - Analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, ELISA, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

SHP-2/PTPN11 Antibody Summary

• Description: Novus Biologicals Goat SHP-2/PTPN11 Antibody (NB100-806) is a polyclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. Anti-SHP-2/PTPN11 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-YENVGLMQQQKSFR corresponding to C-Terminus according to NP_002825.3.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PTPN11
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:28000
  • Western Blot 1 - 3 ug/mL
• Application Notes: WB: Approx. 70 kDa band observed in human skeletal muscle lysate (calculated MW of 68.0 kDa band according to NP_002825.3).
• Control:

  293 Whole Cell Lysate
  Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SHP-2/PTPN11 Antibody

• BPTP3
• EC 3.1.3.48
• MGC14433
• protein tyrosine phosphatase, non-receptor type 11
• protein tyrosine phosphatase-2
• Protein-tyrosine phosphatase 1D
• Protein-tyrosine phosphatase 2C
• PTP1D
• PTP-1D
• PTP2C
• PTP-2C
• PTP2CNS1
• PTPN11
• SHP2
• SHP-2
• SHP2CFC
• SHPTP2
• SH-PTP2
• SH-PTP2Noonan syndrome 1
• SH-PTP3
• tyrosine-protein phosphatase non-receptor type 11

Background

A protein tyrosine phosphatase that is widely expressed and plays a regulatory role in various cell signaling events that are important for many cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SHP-2/PTPN11 Antibody (NB100-806)(1)

Publication using NB100-806

• Saxton TM, Ciruna BG, Holmyard D et al. The SH2 tyrosine phosphatase shp2 is required for mammalian limb development. Nat Genet 2000-04-01 [PMID: 10742110]

Bioinformatics

• Gene Symbol: PTPN11
• Entrez:
  • 5781(Human)
  • 19247(Mouse)
  • 25622(Rat)
• Uniprot:
  • NP_002825.3()