SHP-2/PTPN11 Antibody

Images

 
Western Blot: SHP-2/PTPN11 Antibody [NB100-806] - HEK293 overexpressing PTPN11 and probed with (mock transfection in first lane).
Immunohistochemistry-Paraffin: SHP-2/PTPN11 Antibody [NB100-806] - (3.8ug/ml) staining of paraffin embedded Human Cerebellum Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: SHP-2/PTPN11 Antibody [NB100-806] - Staining (2ug/ml) of Human Muscle lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

SHP-2/PTPN11 Antibody Summary

Immunogen
Peptide with sequence C-YENVGLMQQQKSFR corresponding to C-Terminus according to NP_002825.3.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PTPN11
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 70 kDa band observed in human skeletal muscle lysates and in HEK293 lysates (calculated MW of 68.0 kDa band according to NP_002825.3). In transfected HEK293 transiently expressing PTPN11 bands of approx. 70 kDa band and 49 kDa band are observed. These bands are not observed in the non-transfected HEK293. IHC-P: Human cerebellum shows strong cytoplasm staining in Purkinje cells and processes.
Positive Control
PTPN11/SHP2 Lysate (NBL1-14968)
293 Lysate (NB800-PC6)
Skeletal Muscle Lysate (NB820-59253)
Publications
Read Publication using NB100-806.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SHP-2/PTPN11 Antibody

  • BPTP3
  • EC 3.1.3.48
  • MGC14433
  • protein tyrosine phosphatase, non-receptor type 11
  • protein tyrosine phosphatase-2
  • Protein-tyrosine phosphatase 1D
  • Protein-tyrosine phosphatase 2C
  • PTP1D
  • PTP-1D
  • PTP2C
  • PTP-2C
  • PTP2CNS1
  • PTPN11
  • SHP2
  • SHP-2
  • SHP2CFC
  • SHPTP2
  • SH-PTP2
  • SH-PTP2Noonan syndrome 1
  • SH-PTP3
  • tyrosine-protein phosphatase non-receptor type 11

Background

A protein tyrosine phosphatase that is widely expressed and plays a regulatory role in various cell signaling events that are important for many cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SHP-2/PTPN11 Antibody (NB100-806)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PTPN11
Entrez
Uniprot