SHP-2/PTPN11 Antibody (S08-3G2)

Western Blot: SHP-2/PTPN11 Antibody (S08-3G2) [NBP3-19502] - Western blot detection of SHP-2/PTPN11 in Hela, 293 cell lysates using NBP3-19502 (1:1000 diluted). Predicted band size: 68kDa. Observed band size: 68kDa.

Immunohistochemistry-Paraffin: SHP-2/PTPN11 Antibody (S08-3G2) [NBP3-19502] - Immunohistochemistry of SHP-2/PTPN11 in paraffin-embedded Human breast cancer tissue using NBP3-19502 at dilution 1/50

• Reactivity: Hu
• Applications: WB, IHC
• Clone: S08-3G2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

SHP-2/PTPN11 Antibody (S08-3G2) Summary

• Description: Novus Biologicals Rabbit SHP-2/PTPN11 Antibody (S08-3G2) (NBP3-19502) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human SHP-2/PTPN11 (Uniprot # Q06124)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PTPN11
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1/20
  • Immunohistochemistry-Paraffin
  • Western Blot 1/2000
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for SHP-2/PTPN11 Antibody (S08-3G2)

• BPTP3
• EC 3.1.3.48
• MGC14433
• protein tyrosine phosphatase, non-receptor type 11
• protein tyrosine phosphatase-2
• Protein-tyrosine phosphatase 1D
• Protein-tyrosine phosphatase 2C
• PTP1D
• PTP-1D
• PTP2C
• PTP-2C
• PTP2CNS1
• PTPN11
• SHP2
• SHP-2
• SHP2CFC
• SHPTP2
• SH-PTP2
• SH-PTP2Noonan syndrome 1
• SH-PTP3
• tyrosine-protein phosphatase non-receptor type 11

Background

SHP-2 (also called PTP2C, SH-PTP2, PTP1D, SH-PTP3, Syp and PTPN11) is a protein tyrosine phosphatase (PTP) expressed in most cell types (unlike SHP-1), (1). SHP-2 is involved in t the signal transduction stimulated by EGF, PDGF and Insulin. After cell stimulation, SHP-2 (as well as SHP-1), translocates from cytosol to the plasma membrane and binds to tyrosine-phosphorylated receptors through their SH2 domains, becoming activated in the process (2). When phosphorylated, SHP-2 has been shown to bind Grb2, EGFR, PDGFR, IRS-1 via its SH2 domain (3). Over expression of SHP-2 leads to Juvenile Myelo-monocytic Leukemia and Noonan syndrome (4-5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPN11