SHANK3 Antibody (S69-46)

Images

 
Western Blot: SHANK3 Antibody (S69-46) [NBP1-47610] - analysis of Rat brain membrane lysate showing detection of SHANK3 protein using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46. Load: 15 ug protein. Block: 1.5% ...read more
Immunocytochemistry/ Immunofluorescence: SHANK3 Antibody (S69-46) [NBP1-47610] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-SHANK3 Monoclonal ...read more
Immunohistochemistry-Paraffin: SHANK3 Antibody (S69-46) [NBP1-47610] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody at 1:100 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clone
S69-46
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

SHANK3 Antibody (S69-46) Summary

Immunogen
Synthetic peptide amino acids 840-857 of rat Shank3
Localization
Cytoplasm, Cell Junction, Synapse, Postsynaptic Cell Membrane, Postsynaptic Density
Specificity
Detects approx 190kDa. No cross-reactivity against Shank1 or Shank2.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SHANK3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
Application Notes
1 ug/ml of SHANK3 Antibody was sufficient for detection of Shank3 in 10 ug COS cell lysate transiently transfected with Shank3 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary Antibody.
Publications
Read Publication using NBP1-47610.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for SHANK3 Antibody (S69-46)

  • DEL22q13.3
  • KIAA1650proline rich synapse associated protein 2
  • Proline-rich synapse-associated protein 2
  • prosap2
  • PSAP2SH3 and multiple ankyrin repeat domains protein 3
  • SH3 and multiple ankyrin repeat domains 3
  • shank postsynaptic density protein
  • Shank3
  • SPANK-2

Background

Function: Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.; Subcellular location: Cytoplasm. Cell junction synapse. Cell junction synapse postsynaptic cell membrane postsynaptic density. Note: Postsynaptic density of neuronal cells. Extends into the region subjacent to the PSD.; Tissue specificity: Widely expressed in brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SHANK3 Antibody (NBP1-47610)(1)

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SHANK3
Entrez
Uniprot