![Immunocytochemistry/Immunofluorescence: SH3TC2 Antibody [NBP2-38360] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/SH3TC2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38360-img0003.jpg "Immunocytochemistry/Immunofluorescence: SH3TC2 Antibody [NBP2-38360] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.")
| Reactivity | HuSpecies Glossary |
| Applications | ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit SH3TC2 Antibody - BSA Free (NBP2-38360) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | This antibody was developed against a recombinant protein corresponding to amino acids: SGQVGFVPTRNIDPDSYSPMSRNSAFLSDEERCSLLALGSDKQTECSSFLHTLARTDITSVYRLSGFESIQNPPNDLSASQP |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SH3TC2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. |
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| Control Peptide |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: SH3TC2 Antibody [NBP2-38360] - Staining of human placenta shows weak to moderate membranous and cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/SH3TC2-Antibody-Immunohistochemistry-Paraffin-NBP2-38360-img0006.jpg "Immunohistochemistry-Paraffin: SH3TC2 Antibody [NBP2-38360] - Staining of human placenta shows weak to moderate membranous and cytoplasmic positivity in trophoblastic cells.")
![Immunohistochemistry: SH3TC2 Antibody [NBP2-38360] - Staining of human urinary bladder shows strong cytoplasmic and membranous positivity in urothelial cells.](http://images.novusbio.com/fullsize/SH3TC2-Antibody-Immunohistochemistry-NBP2-38360-img0002.jpg "Immunohistochemistry: SH3TC2 Antibody [NBP2-38360] - Staining of human urinary bladder shows strong cytoplasmic and membranous positivity in urothelial cells.")
![Immunohistochemistry-Paraffin: SH3TC2 Antibody [NBP2-38360] - Staining of human urinary bladder shows strong membranous positivity in urothelial cells.](http://images.novusbio.com/fullsize/SH3TC2-Antibody-Immunohistochemistry-Paraffin-NBP2-38360-img0004.jpg "Immunohistochemistry-Paraffin: SH3TC2 Antibody [NBP2-38360] - Staining of human urinary bladder shows strong membranous positivity in urothelial cells.")
![Immunohistochemistry-Paraffin: SH3TC2 Antibody [NBP2-38360] - Staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/SH3TC2-Antibody-Immunohistochemistry-Paraffin-NBP2-38360-img0005.jpg "Immunohistochemistry-Paraffin: SH3TC2 Antibody [NBP2-38360] - Staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.")
![Immunohistochemistry-Paraffin: SH3TC2 Antibody [NBP2-38360] - Staining of human kidney shows weak membranous and cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/SH3TC2-Antibody-Immunohistochemistry-Paraffin-NBP2-38360-img0007.jpg "Immunohistochemistry-Paraffin: SH3TC2 Antibody [NBP2-38360] - Staining of human kidney shows weak membranous and cytoplasmic positivity in cells in tubules.")
![Western Blot Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) - BSA Free](https://images.novusbio.com/images/Androgen-R-NR3C4-p-Ser213-p-Ser210-Antibody-156C135-2-Western-Blot-NB100-56603-img0004.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")