SGLT2/SLC5A2 Overexpression Lysate (Native)

Images

 
Western Blot: SGLT2 Overexpression Lysate (Adult Normal) [NBL1-16173] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SGLT2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SGLT2/SLC5A2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC224822 and based on accession number NM_003041. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens solute carrier family 5 (sodium/glucose cotransporter), member 2 (SLC5A2), mRNA.
Gene
SLC5A2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
72.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for SGLT2/SLC5A2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SGLT2/SLC5A2 Overexpression Lysate (Native)

  • Low affinity sodium-glucose cotransporter
  • Na(+)/glucose cotransporter 2
  • SGLT2
  • SGLT2sodium/glucose cotransporter 2
  • SLC5A2
  • solute carrier family 5 (sodium/glucose cotransporter), member 2
  • solute carrier family 5 (sodium/glucose transporter), member 2
  • Solute carrier family 5 member 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-20338
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NB110-39113
Species: Hu, Mu, Rt
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-22218
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-82862
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB120-2941
Species: Hu, Mu, Rt, Bv, Pm
Applications: WB, IHC, IHC-P
H00000265-M03
Species: Hu
Applications: WB, ELISA
NBP1-33596
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-69565
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-92005
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-89680
Species: Hu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-49533
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-47778
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF4090
Species: Hu
Applications: WB, IHC, IP, Neut
NBP1-76847
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P

Publications for SGLT2/SLC5A2 Lysate (NBL1-16173) (0)

There are no publications for SGLT2/SLC5A2 Lysate (NBL1-16173).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SGLT2/SLC5A2 Lysate (NBL1-16173) (0)

There are no reviews for SGLT2/SLC5A2 Lysate (NBL1-16173). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SGLT2/SLC5A2 Lysate (NBL1-16173) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional SGLT2/SLC5A2 Products

Bioinformatics Tool for SGLT2/SLC5A2 Lysate (NBL1-16173)

Discover related pathways, diseases and genes to SGLT2/SLC5A2 Lysate (NBL1-16173). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SGLT2/SLC5A2 Lysate (NBL1-16173)

Discover more about diseases related to SGLT2/SLC5A2 Lysate (NBL1-16173).
 

Pathways for SGLT2/SLC5A2 Lysate (NBL1-16173)

View related products by pathway.

PTMs for SGLT2/SLC5A2 Lysate (NBL1-16173)

Learn more about PTMs related to SGLT2/SLC5A2 Lysate (NBL1-16173).
 

Research Areas for SGLT2/SLC5A2 Lysate (NBL1-16173)

Find related products by research area.

Blogs on SGLT2/SLC5A2

There are no specific blogs for SGLT2/SLC5A2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Glut1 Antibody
NB110-39113

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SGLT2/SLC5A2 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol SLC5A2
Entrez