SF3B4 Antibody (3A1)

Western Blot: SF3B4 Antibody (3A1) [NBP1-92692] - Analysis of different cell lysates, cytosol or nuclear enriched fractions, using mouse mAb to splicing factor SF3B4, NBP1-92692, dilution 1:1,000 in green: [1] protein standard (red), [2] NIH-3T3 cytosolic fraction [3] NIH-3T3 nuclear fraction [4] HeLa cytosolic and [5] HeLa nuclear fractions. Strong single band at 49kDa represents the SF3B4 protein, which is expressed exclusively in the nuclei. The same blot was simultaneously probed with rabbit pAb to GAPDH, dilution 1:20,000, in red. The 37kDa band corresponds to GAPDH protein, detected mainly in the cytosolic fractions of these cells.

Immunocytochemistry/Immunofluorescence: SF3B4 Antibody (3A1) [NBP1-92692] - Analysis of HeLa cells stained with mouse mAb to splicing factor SF3B4, NBP1-92692, dilution 1:1,000 in red, and costained with chicken pAb to vimentin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. The NBP1-92692 antibody reveals strong granular staining of the nuclei, while the vimentin antibody specifically labels cytoplasmic intermediate filaments.

Immunocytochemistry/Immunofluorescence: SF3B4 Antibody (3A1) [NBP1-92692] - HeLa cells stained with NBP1-92692 (red), chicken antibody to Vimentin (NB300-223, green) and DNA (blue, stained with DAPI). The SF3B4 (3A1) antibody reveals strong granular nuclear staining which is a little different from the DNA stain and presumably reflects splicosomal complexes. The Vimentin antibody stains the cytoplasmic intermediate filament network of the HeLa cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: 3A1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

SF3B4 Antibody (3A1) Summary

• Immunogen: Full length recombinant human SF3B4 expressed in and purified from E. coli. [UniProt# Q15427]
• Localization: Nucleus.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SF3B4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:1000
  • Western Blot 1:1000
• Application Notes: This SF3B4 (3A1) antibody is useful for Immunocytochemistry/Immunofluorescence, immunohistochemistry, and Western blot, where a band can be seen at approximately 49 kDa.
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50% PBS, 50% glycerol
• Preservative: 5mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for SF3B4 Antibody (3A1)

• Hsh49
• pre-mRNA splicing factor SF3b 49 kDa subunit
• Pre-mRNA-splicing factor SF3b 49 kDa subunit
• SAP 49
• SAP49MGC10828
• SF3b49
• SF3b50
• spliceosomal protein
• spliceosome-associated protein (U2 snRNP)
• Spliceosome-associated protein 49
• splicing factor 3B subunit 4
• splicing factor 3b, subunit 4, 49kD
• splicing factor 3b, subunit 4, 49kDa

Background

SF3B4 (splicing factor 3B subunit 4, also known as SF3b50 or SAP49), is a ubiquitously expressed nuclear protein which forms a subunit of splicing factor SF3B (SF3B1/SAP155/SF3B155, SF3B2/SAP145/SF3B145, SF3B3/SAP130/SF3B130, SF3B4/SAP49/SF3B49, SF3B14A, PHF5A/SF3B14B, SF3B10 and SF3B125) required for 'A' complex assembly formed by the stable binding of U2 snRNP to pre-mRNA's BPS (branchpoint sequence). SF3B4 interacts directly with SF3B2 and also forms a component of U11/U12 snRNPs that are a part of the U12-type spliceosome. SF3B4 has also been suggested to implicate in 'E' complex assembly and is also found in 'B' and 'C' complexes. SF3B4 is also associated with minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. Defects in SF3B4 have been linked to AFD1 (acrofacial dysostosis type 1) which is a form of acrofacial dysostosis, a group of disorders characterized by malformation of the craniofacial skeleton and limbs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SF3B4 Antibody (NBP1-92692)(1)

Publication using NBP1-92692

• Sikorski K, Mehta A et al. A high-throughput pipeline for validation of antibodies. Nat Methods 2018-01-11 [PMID: 30377371] (Human)
  
  Details:
  Antibody validation based on denaturing gel electrophoresis of biotinylated cell lysates (PAGE) followed by mass spectrometry (MS) and antibody array analysis (MAP).

Bioinformatics

• Gene Symbol: SF3B4
• Entrez:
  • 10262(Human)
• Uniprot:
  • Q15427(Human)