SAP130 Antibody

Western Blot: SAP130 Antibody [NB100-1077] - Staining (0.1ug/ml) of nuclear lysate from HeLa cells (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, IHC, IHC-Fr, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

SAP130 Antibody Summary

• Immunogen: Peptide with sequence C-VSKKLEDIRTRYAF corresponding to C-Terminus according to NP_036558.3.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SF3B3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 150 kDa band observed in HeLa nuclear lysates (calculated MW of 136 kDa band according to NP_036558.3). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 23594796). Use in ELISA reported in scientific literature (PMID: 22564223).
• Theoretical MW: 136 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  SAP130 Overexpression Lysate

• Control Peptide:

  SAP130 Peptide (NB100-1077PEP)

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SAP130 Antibody

• KIAA0017splicing factor 3b, subunit 3, 130kD
• Pre-mRNA-splicing factor SF3b 130 kDa subunit
• RSE1
• SAP130SAP 130
• SF3B130
• SF3b130pre-mRNA splicing factor SF3b, 130 kDa subunit
• Spliceosome-associated protein 130
• splicing factor 3B subunit 3
• splicing factor 3b, subunit 3, 130kDa
• STAF130

Background

This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SAP130 Antibody (NB100-1077)(3)

Publications using NB100-1077

• Matsushita K, Tamura M, Tanaka N et al. Interactions between SAP155 and FUSE-binding protein-interacting repressor bridges c-Myc and P27Kip1 expression. Mol Cancer Res. 2013-07-01 [PMID: 23594796] (IHC-Fr, WB, Human)
• Naylor, M C, Lazar, D A, Zamora, I J, Mushin, O P, Yu, L, Brissett, A E Olutoye, O O. Increased in vitro differentiation of fibrocytes from keloid patients is inhibited by serum amyloid P. Wound Repair and Regeneration, 20: 277-283. doi: 10.1111/j.1524-475X.2012.00782.x. 2012-01-01 [PMID: 22564223] (ELISA, Human)
• Das BK, Xia L, Palandjian L et al. Characterization of a protein complex containing spliceosomal proteins SAPs 49, 130, 145, and 155. Mol Cell Biol 1999-10-01 [PMID: 10490618]

Review for SAP130 Antibody (NB100-1077) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Paramananda Saikia WB Human 07/02/2014

Summary

• Application: Western Blot
• Sample Tested: Cell lysate
• Species: Human
• Lot: 309065543

Bioinformatics

• Gene Symbol: SF3B3
• Entrez:
  • 23450(Human)
  • 101943(Mouse)
• Uniprot:
  • Q15393(Human)