SAP130 Antibody

Images

 
Western Blot: SAP130 Antibody [NB100-1077] - Staining (0.1ug/ml) of nuclear lysate from HeLa cells (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

SAP130 Antibody Summary

Immunogen
Peptide with sequence C-VSKKLEDIRTRYAF corresponding to C-Terminus according to NP_036558.3.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SF3B3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Peptide ELISA Detection limit 1:2000
Application Notes
WB: Approx. 150 kDa band observed in HeLa nuclear lysates (calculated MW of 136 kDa band according to NP_036558.3).
Theoretical MW
136 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
SAP130 Lysate (NBL1-15877)
Reviewed Applications
Read 1 Review rated 5
using
NB100-1077 in the following applications:

Publications
Read Publications using
NB100-1077 in the following applications:

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SAP130 Antibody

  • KIAA0017splicing factor 3b, subunit 3, 130kD
  • Pre-mRNA-splicing factor SF3b 130 kDa subunit
  • RSE1
  • SAP130SAP 130
  • SF3B130
  • SF3b130pre-mRNA splicing factor SF3b, 130 kDa subunit
  • Spliceosome-associated protein 130
  • splicing factor 3B subunit 3
  • splicing factor 3b, subunit 3, 130kDa
  • STAF130

Background

This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SAP130 Antibody (NB100-1077)(3)

Review for SAP130 Antibody (NB100-1077) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-1077:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 07/02/2014
View

Summary

ApplicationWestern Blot
Sample TestedCell lysate
SpeciesHuman
Lot309065543
CommentsThe antibody worked excellent.

Blocking

Blocking Details5% BSA in TBST

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionDonkey anti-goat

Details

Detection NotesLuminescence

Comments

CommentsThe antibody worked excellent.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SAP130 Antibody (NB100-1077) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for SAP130 Antibody (NB100-1077)

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Blogs on SAP130

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Recent Reviews

5
5
1
4
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2
0
1
0

 
Anonymous
07/02/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol SF3B3
Entrez
Uniprot