SF3B2 Antibody

Images

 
Western Blot: SF3B2 Antibody [NB100-79847] - Detection of Human SF3b145/SAP145 on HeLa whole cell lysate using NB100-79847. SF3b145/SAP145 was also immunoprecipitated by rabbit anti-SF3b145/SAP145 antibody NB100-79848.
Immunohistochemistry: SF3B2 Antibody [NB100-79847] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- SF3b145/SAP145 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Western Blot: SF3B2 Antibody [NB100-79847] - Analysis of SF3B2 in HeLa whole cell lysate using anti- SF3B2 antibody. The primary antibody was used at a dilution of 1:500 with overnight incubation at 4C. Image from ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

SF3B2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 775 and 825 of human splicing factor 3b, subunit 2, 145kDa (spliceosome associated protein 145) using the numbering given in entry AAA97461.1 (GeneID 10992).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SF3B2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:100000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 mcg/mg lysate
Application Notes
Immunohistochemistry 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Reviewed Applications
Read 1 Review rated 4
using
NB100-79847 in the following applications:

Publications
Read Publication using
NB100-79847 in the following applications:

  • IP
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SF3B2 Antibody

  • Cus1
  • pre-mRNA splicing factor SF3b 145 kDa subunit
  • Pre-mRNA-splicing factor SF3b 145 kDa subunit
  • SAP145SAP 145
  • SF3b1
  • SF3B145
  • SF3b150
  • spliceosome associated protein 145
  • Spliceosome-associated protein 145
  • splicing factor 3B subunit 2
  • splicing factor 3b, subunit 2, 145kD
  • splicing factor 3b, subunit 2, 145kDa

Background

This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-92692
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ICC/IF
H00171023-M05
Species: Hu
Applications: WB, ELISA
NB100-1077
Species: Hu
Applications: WB, PEP-ELISA
NBP1-19120
Species: Hu, Mu
Applications: WB, IHC-P, IP
NBP1-89827
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IF
NBP1-87769
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB100-1774
Species: Hu, Rt, Am, Dr
Applications: WB, EM, ELISA, ICC/IF, IP
NB200-106
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-87431
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-82454
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-91776
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-71687
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
AF2065
Species: Mu
Applications: WB, Simple Western, ICC
NBP1-80965
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-37507
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
NBP2-16035
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB120-13888
Species: Hu, Mu
Applications: WB, ChIP, Flow, ICC/IF, IHC-P, CyTOF-ready, IHC-WhMt
NBP1-47804
Species: Hu, Mk
Applications: WB, ICC/IF, IHC, IHC-P
AF2988
Species: Mu, Rt
Applications: WB

Publications for SF3B2 Antibody (NB100-79847)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IP.


Filter By Application
IP
(1)
All Applications
Filter By Species
Human
(1)
All Species

Review for SF3B2 Antibody (NB100-79847) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-79847:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot SF3B2 NB100-79847
Enlarge
reviewed by:
Anonymous
WB Human 10/16/2015
View

Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman
LotA1

Blocking

Blocking DetailsBlocker=Skimmed milk, Buffer= 1XTris Buffer Saline+0.1 % Tween 20, 2hrs at RT

Primary Anitbody

Dilution Ratio1:500 over night at 4 degree Celsius

Secondary Antibody

Secondary Descriptionanti-rabbit IgG, HRP
Secondary Manufacturer Cat#7074P2
Secondary Concentration1:20,000

Details

Detection Notes30 seconds exposure

Comments

CommentsSURE IT WORKED FOR ME !

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SF3B2 Antibody (NB100-79847) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional SF3B2 Products

Bioinformatics Tool for SF3B2 Antibody (NB100-79847)

Discover related pathways, diseases and genes to SF3B2 Antibody (NB100-79847). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SF3B2 Antibody (NB100-79847)

Discover more about diseases related to SF3B2 Antibody (NB100-79847).
 

Pathways for SF3B2 Antibody (NB100-79847)

View related products by pathway.

PTMs for SF3B2 Antibody (NB100-79847)

Learn more about PTMs related to SF3B2 Antibody (NB100-79847).

Blogs on SF3B2

There are no specific blogs for SF3B2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
10/16/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol SF3B2
Entrez
Uniprot