SF3B2 Antibody

Western Blot: SF3B2 Antibody [NB100-79847] - Detection of Human SF3b145/SAP145 on HeLa whole cell lysate using NB100-79847. SF3b145/SAP145 was also immunoprecipitated by rabbit anti-SF3b145/SAP145 antibody NB100-79848.

Immunohistochemistry: SF3B2 Antibody [NB100-79847] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- SF3b145/SAP145 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Western Blot: SF3B2 Antibody [NB100-79847] - Analysis of SF3B2 in HeLa whole cell lysate using anti- SF3B2 antibody. The primary antibody was used at a dilution of 1:500 with overnight incubation at 4C. Image from verified customer review.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

SF3B2 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 775 and 825 of human splicing factor 3b, subunit 2, 145kDa (spliceosome associated protein 145) using the numbering given in entry AAA97461.1 (GeneID 10992).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SF3B2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 mcg/mg lysate
  • Western Blot 1:2000-1:100000
• Application Notes: Immunohistochemistry 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SF3B2 Antibody

• Cus1
• pre-mRNA splicing factor SF3b 145 kDa subunit
• Pre-mRNA-splicing factor SF3b 145 kDa subunit
• SAP145SAP 145
• SF3b1
• SF3B145
• SF3b150
• spliceosome associated protein 145
• Spliceosome-associated protein 145
• splicing factor 3B subunit 2
• splicing factor 3b, subunit 2, 145kD
• splicing factor 3b, subunit 2, 145kDa

Background

This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SF3B2 Antibody (NB100-79847)(3)

Publications using NB100-79847

• Pappas G, Munk SHN, Watanabe K, Thomas Q et Al. MDC1 maintains active elongation complexes of RNA polymerase II Cell Rep 2023-01-14 [PMID: 36640322]
• Pawellek A, Ryder U, Tammsalu T, King LJ et Al. Characterisation of the biflavonoid hinokiflavone as a pre-mRNA splicing modulator that inhibits SENP Elife 2017-09-09 [PMID: 28884683]
• Tresini M, Warmerdam DO, Kolovos P et al. The core spliceosome as target and effector of non-canonical ATM signalling. Nature 2015-07-02 [PMID: 26106861] (IP, Human)

Review for SF3B2 Antibody (NB100-79847) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 10/16/2015

Summary

• Application: Western Blot
• Sample Tested: Hela whole cell lysate
• Species: Human
• Lot: A1

Bioinformatics

• Gene Symbol: SF3B2
• Entrez:
  • 10992(Human)
• Uniprot:
  • Q13435(Human)