SETDB1 Antibody (5H6A12)

Western Blot: SETDB1 Antibody (5H6A12) [NBP1-51677] - Analysis using SETDB1 mouse mAb against MCF-7 (1),T47D (2), HEK293 (3), JURKAT (4), NIH/3T3 (5), F9 (6), RAW246.7 (7) and Cos7 (8) cell lysate.

Immunocytochemistry/Immunofluorescence: SETDB1 Antibody (5H6A12) [NBP1-51677] - Analysis of LOVO cells using SETDB1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

• Reactivity: Hu, Mu, Pm
• Applications: WB, ELISA, ICC/IF
• Clone: 5H6A12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

SETDB1 Antibody (5H6A12) Summary

• Immunogen: Purified recombinant fragment of human SETDB1 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SETDB1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 180 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for SETDB1 Antibody (5H6A12)

• ERG-associated protein with SET domain
• ESETEC 2.1.1.43
• H3-K9-HMTase 4
• Histone H3-K9 methyltransferase 4
• histone-lysine N-methyltransferase SETDB1
• histone-lysine N-methyltransferase, H3lysine-9 specific 4
• KG1T
• KIAA0067H3-K9-HMTase4
• KMT1EERG-associated protein with a SET domain, ESET
• Lysine N-methyltransferase 1E
• SET domain bifurcated 1
• SET domain, bifurcated 1

Background

The SET domain is a highly conserved, approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger conserved region present in the Drosophila Trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and 'Enhancer of zeste' proteins, from which the acronym SET is derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for SETDB1 Antibody (NBP1-51677). (Showing 1 - 1 of 1 FAQ).

1. Could you please let me know the location of the immunogen in SETDB1(1291 amino acids) for antibody NBP1-51677 {SETDB1 Antibody (5H6A12)}?
   • The immunogen fragment consists of amino acids 868-1211.

Bioinformatics

• Gene Symbol: SETDB1
• Entrez:
  • 9869(Human)
• Uniprot:
  • Q15047(Human)