SETD8 Antibody - Azide and BSA Free

Western Blot: SETD8 Antibody [NBP2-99864] - Anti-SETD8 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: HCT-116 Whole Cell Lysate. Lane C: U87-MG Whole Cell Lysate. Lane D: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 50 kDa

Immunoprecipitation: SETD8 Antibody [NBP2-99864] - SETD8 was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. 4 ul anti-SETD8 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-SETD8 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 54 kDa

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SETD8 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human SETD8 (Accession#: NP_065115.3; Lys195-His352)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KMT5A
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunoprecipitation 5-10 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for SETD8 Antibody - Azide and BSA Free

• H4-K20-HMTase KMT5A
• H4-K20-HMTase SETD8
• H4K20-specific histone methyltransferase splice variant Set8b
• Histone-lysine N-methyltransferase SETD8
• KMT5A SET domain-containing protein 8
• KMT5A
• Lysine N-methyltransferase 5A
• PR/SET domain containing protein 8
• PR/SET domain-containing protein 07
• PR/SET07
• PRSET7
• PR-Set7
• PR-Set7EC 2.1.1.43
• SET domain containing (lysine methyltransferase) 8
• SET07
• SET07SET8H4-K20-specific histone methyltransferase
• SET8
• SETD8

Background

Histone methyltransferase that specifically monomethylates 'Lys-20' of histone H4. H4 'Lys-20'monomethylation is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression.Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes.Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNAduring mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substratecompared to free histones

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KMT5A