SETD7/9 Antibody - Azide and BSA Free

Western Blot: SETD7/9 Antibody [NBP2-99744] - Anti-SETD7/9 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 48 kDa. Observed band size: 53 kDa (We are unsure as to the identity of these extra bands).

Immunoprecipitation: SETD7/9 Antibody [NBP2-99744] - SETD7/9 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. 2 ul anti-SETD7/9 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-SETD7/9 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 52 kDa

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SETD7/9 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human SETD7/9 (Accession#: NP_085151.1; Asp2-Lys366)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SETD7
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunoprecipitation 1-4 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for SETD7/9 Antibody - Azide and BSA Free

• EC 2.1.1.43
• H3-K4-HMTase SETD7
• histone H3-lysine 4-specific methyltransferase
• histone-lysine N-methyltransferase SETD7
• KIAA1717SET domain-containing protein 7
• KMT7FLJ21193
• Lysine N-methyltransferase 7
• SET domain containing (lysine methyltransferase) 7
• SET7/9Histone H3-K4 methyltransferase SETD7
• SET7Set9
• SET9

Background

Diverse signal transduction pathways impinging on the N-terminal tails of histones lead to a number of post-translational modifications including acetylation, phosphorylation, poly(ADP-ribosylation), ubiquitination and methylation. These modifications play critical roles in regulating chromatin structure and gene expression. Histone methyltransferases (HMTases) selectively methylate evolutionarily conserved arginine or lysine residues, primarily in the N-terminal tails of histones H3 and H4. Set7/9 is a histone specific HMTase that methylates histone H3 lysine 4. Set7/9 transfers methyl groups to lysine 4 of histone H3 in complex with S-adenosyl-L-methionine. Human Set7/9 is a 336 amino acid protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SETD7/9 Antibody (NBP2-99744)(1)

Publication using NBP2-99744

• Li G, Li D, Wu C et al. Homocysteine-targeting compounds as a new treatment strategy for diabetic wounds via inhibition of the histone methyltransferase SET7/9 Experimental & molecular medicine Jul 20 2022 [PMID: 35859119] (IP, Human)

Bioinformatics

• Gene Symbol: SETD7