Serpin F2/alpha 2-Antiplasmin Antibody [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB, IP, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

Serpin F2/alpha 2-Antiplasmin Antibody [Alexa Fluor® 647] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Serpin F2/α₂‑Antiplasmin
  Met28-Lys491
  Accession # P08697
• Specificity: Detects human Serpin F2/ alpha ₂‑Antiplasmin in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross‑reactivity with recombinant mouse (rm) Serpin F2 is observed and less than 1% cross-react
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Neutralization
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin F2/alpha 2-Antiplasmin Antibody [Alexa Fluor® 647]

• AAPclade F (alpha-2 antiplasmin
• alpha 2-Antiplasmin
• Alpha-2-AP
• Alpha-2-PI
• Alpha-2-plasmin inhibitor
• pigment epithelium derived factor), member 2
• Serpin F2
• serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epitheliumderived factor), member 2

Background

Serpin F2 is a member of the Serpin superfamily and the primary physiological inhibitor of the serine protease plasmin, which is responsible for the dissolution of fibrin clots (1, 2). In addition to plasmin, Serpin F2 is also an efficient inhibitor of trypsin and chymotrypsin (3). Liver and kidney are major sites of Serpin F2 production and other tissues such as muscle, intestine, central nervous system, and placenta also express its mRNA at a moderate level. The tissue expression pattern of Serpin F2 indicates that it is a key regulator of plasmin-mediated proteolysis in these tissues (4). Human Serpin F2 is synthesized as a 491 amino acid precursor with a 27 amino acid signal peptide. The secreted protein has a short propeptide (residues 28‑39) and a mature chain (residues 40‑491). The presence of the propeptide did not affect its ability to inhibit plasmin but reduced its cross-linking ability to fibrin (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.