Serpin E1/PAI-1 Antibody Pair [HRP]

Sandwich ELISA: Serpin E1/PAI-1 Antibody Pair [HRP] [NBP2-79560] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP
• Standard Curve Range: 46.88-3000 pg/ml (example only; lot dependent)
• Sensitivity: 46.88 pg/ml (example only; lot dependent)

Serpin E1/PAI-1 Antibody Pair [HRP] Summary

• Description: Solid Phase sandwich ELISA for the quantitative determination of Human Serpin E1/PAI-1.
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Serpin E1/PAI-1.
• Standard Curve Range: 46.88-3000 pg/ml (example only; lot dependent)
• Sensitivity: 46.88 pg/ml (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Sample Volume: 100 ul
• Gene: SERPINE1

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Serpin E1/PAI-1 Antibody Pair [HRP]

• Endothelial plasminogen activator inhibitor
• Nexin
• PAI1
• PAI-1
• PAI1PAI-1
• PAISerpin E1
• PLANH1
• PLANH1plasminogen activator inhibitor 1
• serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1
• Serpin E1
• serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1

Background

PAI-1 and PAI-2 (for plasminogen activator inhibitor-1 and -2) are members of the serpin serine proteinase inhibitor family. PAI-1 and PAI-2 have been shown to regulate uPA (urokinase-type plasminogen activator) and tPA (tissue plasminogen activator), resulting in the inhibition of proteolytic activity. Members of the serpin family generally complex with their target proteinases, then disassociate slowly into cleaved species that fold into stable inactive forms. PAI-1 can fold into the inactive state without cleavage, resulting in the latent form of PAI-1. Activity can be restored to the latent form of PAI-1 through denaturation and renaturation. PAI-2 occurs in secreted and cytosolic forms through facultative polypeptide translocation. uPA is a serine proteinase that is a member of the trypsin family. It is responsible for the cleavage of plasminogen at the Arg-Val bond to produce plasmin. uPA consists of two chains designated A and B. The A chain can be cleaved, resulting in low and high molecular mass forms of uPA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: SERPINE1