Serpin B5/Maspin Antibody [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, PC-3 human prostate cancer cell line, and RT-4 human bladder carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Serpin B5/Maspin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2218) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Serpin B5/Maspin at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Serpin B5/Maspin knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Serpin B5/Maspin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2218) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Serpin B5/Maspin at approximately 40 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Serpin B5/Maspin Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Serpin B5/Maspin
  Met1-Pro375
  Accession # P36952
• Specificity: Detects human Serpin B5/Maspin in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Serpin A1, rhSerpin A3, rhSerpin A4, rhSerpin A5, rhSerpin C1, rhSerpin F2, rhSerpin I1, rhSerpin I2, rhPEDF, and recombinant mouse Serpin D1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SERPINB5
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin B5/Maspin Antibody [Unconjugated]

• Maspin
• Peptidase inhibitor 5
• PI5
• PI-5
• PI5protease inhibitor 5 (maspin)
• serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5
• Serpin B5
• serpin peptidase inhibitor, clade B (ovalbumin), member 5

Background

Serpin B5, also known as Maspin, is a secreted member of the Serpin family although it has no known serine protease inhibitor activity. It is produced by mammary epithelial cells and functions as a tumor suppressor. Serpin B5 is a valuable biomarker of tumor progression and overall prognosis. The amino acid sequence of human Serpin B5 is 99%, 92%, 89%, 88% and 64% identical to that of chimpanzee, dog, mouse, rat and chicken.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Serpin B5/Maspin Antibody (AF2218)(1)

Publication using AF2218

• Bass R, Wagstaff L, Ravenhill L, Ellis V Binding of extracellular maspin to beta1 integrins inhibits vascular smooth muscle cell migration. J. Biol. Chem., 2009-07-28;284(40):27712-20. 2009-07-28 [PMID: 19638634] (Immunoprecipitation, Western Blot, Human)

Bioinformatics

• Gene Symbol: SERPINB5
• Uniprot:
  • P36952()