Serpin A4/Kallistatin Antibody

Images

 
Western Blot: Kallistatin Antibody [NBP2-17013] - Sample (30 ug of whole cell lysate) A: HCT116 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Kallistatin Antibody [NBP2-17013] - Immunofluorescence analysis of methanol-fixed HCT116, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Kallistatin Antibody [NBP2-17013] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:250 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Serpin A4/Kallistatin Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Kallistatin. The exact sequence is proprietary.
Localization
Secreted
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SERPINA4
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Serpin A4/Kallistatin Antibody

  • antitrypsin), member 4
  • KAL
  • Kallistatin
  • KLST
  • KSTKallikrein inhibitor
  • member 4
  • PI-4
  • PI4Peptidase inhibitor 4
  • protease inhibitor 4 (kallistatin)
  • serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
  • Serpin A4
  • serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)

Background

Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SERPINA4
Entrez
Uniprot