Serpin A4/Kallistatin Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Serpin A4/Kallistatin Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Serpin A4/Kallistatin
  Gln21-Pro427
  Accession # AAA59454
• Specificity: Detects human Serpin A4/Kallistatin in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross‑reactivity with recombinant human (rh) Serpin A1, rhSerpin A3, rhSerpin A5, rhSerpin C1, rhSerpin D1, rhSerpin E1, rhSerpin E2, and rhSerpin F2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SERPINA4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human Serpin A4/Kallistatin Antibody (Catalog # MAB16691)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin A4/Kallistatin Antibody [Biotin]

• antitrypsin), member 4
• KAL
• Kallistatin
• KLST
• KSTKallikrein inhibitor
• member 4
• PI-4
• PI4Peptidase inhibitor 4
• protease inhibitor 4 (kallistatin)
• serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
• Serpin A4
• serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)

Background

Serpin A4 is a member of the Serpin superfamily of the serine protease inhibitors (1). Known also as Kallistatin or proteinase inhibitor 4, Serpin A4 has been shown to function as a specific inhibitor of tissue kallikrein and as an inhibitor of angiogenesis and tumor growth (2, 3).

1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
2. Chen, V.C. et al. (2000) J. Biol. Chem. 275:40371.
3. Miao, R.Q. et al. (2002) Blood 100:3245.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SERPINA4
• Uniprot:
  • AAA59454()