Serine Palmitoyltransferase 2 Antibody

Western Blot: Serine Palmitoyltransferase 2 Antibody [NBP2-20308] - Serine Palmitoyltransferase 2 levels in human neuroblastoma cells. Cells were untreated or treated with various drugs. Image from verified customer review.

Immunohistochemistry-Paraffin: Serine Palmitoyltransferase 2 Antibody [NBP2-20308] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.

Western Blot: Serine Palmitoyltransferase 2 Antibody [NBP2-20308] - Sample (30 ug of whole cell lysate) A: IMR32 7. 5% SDS PAGE gel, diluted at 1:1000.

Western Blot: Serine Palmitoyltransferase 2 Antibody [NBP2-20308] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 7. 5% SDS PAGE gel, diluted at 1:500.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Serine Palmitoyltransferase 2 Antibody Summary

• Description: Novus Biologicals Rabbit Serine Palmitoyltransferase 2 Antibody (NBP2-20308) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Serine Palmitoyltransferase 2. The exact sequence is proprietary.
• Localization: Endoplasmic reticulum membrane; Single-pass membrane protein
• Predicted Species: Rat (95%), Bovine (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPTLC2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Application Notes: Serine Palmitoyltransferase 2 antibody validated for WB from a verified customer review.
• Theoretical MW: 63 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (84%), Xenopus laevis (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Serine Palmitoyltransferase 2 Antibody

• EC 2.3.1
• EC 2.3.1.50
• KIAA0526
• KIAA0526HSN1C
• LCB 2
• LCB2
• LCB2a
• LCB2NSAN1C
• Long chain base biosynthesis protein 2
• Long chain base biosynthesis protein 2a
• Serine Palmitoyltransferase 2
• serine palmitoyltransferase, long chain base subunit 2
• serine palmitoyltransferase, subunit II
• Serine-palmitoyl-CoA transferase 2
• SPT 2
• SPT2
• SPTLC2

Background

This gene encodes a long chain base subunit of serine palmitoyltransferase. Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine. Mutations in this gene were identified in patients with hereditary sensory neuropathy type I. Alternatively spliced variants encoding different isoforms have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SPTLC2
• Uniprot:
  • O15270(Human)