Separase Antibody [DyLight 405]

Product Discontinued

Separase Antibody [DyLight 405] Summary

• Immunogen: Synthetic peptide made to an internal portion of human Separase (within residues 1400-1500). [Swiss-Prot# Q14674]
• Localization: Cytoplasm and Nucleus.
• Specificity: This detects both isoforms of Separase.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ESPL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This Separase antibody is useful for Western blot, where bands are seen at ~230 kDa and ~66 kDa.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Separase Antibody [DyLight 405]

• Caspase-like protein ESPL1
• EC 3.4.22.49
• ESP1extra spindle poles like 1
• extra spindle pole bodies homolog 1 (S. cerevisiae)
• extra spindle poles like 1 (S. cerevisiae)
• Extra spindle poles-like 1 protein
• FLJ46492
• KIAA0165separin
• Separase

Background

In normal cellular proliferation, the maintenance of constant centriole numbers is strongly associated with cell cycle process and the disengagement of mother-daugther centriole (a requirement for duplication of centriole) is facilitated by separase (ESPL1, a cysteine endopeptidase) mediated proteolytic cleavage of cohesin, a "glue" protein complex which is also essential to sister-chromatide cohesion. Separase is a caspase-like protease which plays a key role in chromosome segregation by cleaving SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle phases, separase is kept inactivated, and its proteolytic activity is tightly regulated by multiple inhibitory mechanisms combining securin binding, CyclinB1/Cdk1 mediated serine residue phosphorylation (pSer1226), PP2A binding and autocatalytic cleavage. Ectopic activation of separase proteolytic activity results in premature sister-chromatide separation/centriole disengagement and separase overexpression has been shown to induce aneuploidy and carcinogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ESPL1