SENP2 Antibody

Immunohistochemistry-Paraffin: SENP2 Antibody [NBP1-31217] - Cal27 xenograft, using SENP2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: SENP2 Antibody [NBP1-31217] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SENP2 antibody [N1], N-term (NBP1-31217) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

SENP2 Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human SENP2. The exact sequence is proprietary.
• Localization: Nucleus, nuclear pore complex, Nucleus membrane, Peripheral membrane protein, Nucleoplasmic side, Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SENP2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol (pH7)
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for SENP2 Antibody

• AXAM2
• AXAM2Axam2
• DKFZp762A2316
• EC 3.4.22
• EC 3.4.22.68
• KIAA1331sentrin-specific protease 2
• SENP2
• Sentrin/SUMO-specific protease SENP2
• SMT3IP2
• SMT3IP2EC 3.4.22.-
• SMT3-specific isopeptidase 2
• SUMO1/sentrin/SMT3 specific peptidase 2
• SUMO1/sentrin/SMT3 specific protease 2

Background

Covalent attachment of one protein to another is one of the more prominent posttranslational modifications in respect to size and ubiquity. Ubiquitin is the most familiar of the protein modifiers and its activation and transfer to target proteins has been studied extensively. Recently, a new group of ubiquitin-like proteins has been receiving a lot of attention. SUMO, or Sentrin, is one of the most intriguing. SUMO has been implicated in the stabilization of target proteins and/or their localization to sub-cellular complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for SENP2 Antibody (NBP1-31217)(3)

Publications using NBP1-31217

• Nguyen MTH, Imanishi M, Li S et al. Endothelial activation and fibrotic changes are impeded by laminar flow-induced CHK1-SENP2 activity through mechanisms distinct from endothelial-to-mesenchymal cell transition Frontiers in Cardiovascular Medicine 2023-08-30 [PMID: 37711550] (Western Blot, Human)
• Gao X, Wu Y, Qiao L, Feng X. SENP2 suppresses NF-kB activation and sensitizes breast cancer cells to doxorubicin Eur. J. Pharmacol. 2019-03-30 [PMID: 30940449] (WB, Human)
• Paez-Mayorga J, Chen AL, Kotla S et al. Ponatinib Activates an Inflammatory Response in Endothelial Cells via ERK5 SUMOylation. Front Cardiovasc Med 2018-09-06 [PMID: 30238007] (WB, Human)

Bioinformatics

• Gene Symbol: SENP2