Senataxin Antibody - BSA Free

Western Blot: Senataxin Antibody [NB100-57543] - Detection of Human Senataxin by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Senataxin antibody NB100-57543 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: Senataxin Antibody [NB100-57543] - Detection of human Senataxin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Senataxin antibody NB100-57543 used for IP at 6 ug per reaction. Senataxin was also immunoprecipitated by rabbit anti-Senataxin antibody NB100-57542. For blotting immunoprecipitated Senataxin, NB100-57543 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu
• Applications: WB, IP, ChIP, KO, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Senataxin Antibody - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit Senataxin Antibody - BSA Free (NB100-57543) is a polyclonal antibody validated for use in WB, IP and ChIP. Anti-Senataxin Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 2627 and 2677 of human senataxin (NP_055861.2).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SETX
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Knockdown Validated
  • Knockout Validated
  • Western Blot 1:2000 - 1:10000
• Application Notes: Use in chromatin immunoprecipitation reported in scientific literature (PMID 26700805).
• Theoretical MW: 302 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Senataxin Antibody - BSA Free

• ALS4recessive, non-Friedreich type 1
• AOA2FLJ43459
• DKFZp781B151
• KIAA0625FLJ12840
• senataxin
• SETX

Background

Mutations in Senataxin result in the neurodegenerative disorders Ataxia-Oculomotor Apraxia 2 (AOA2) and amyotrophic lateral sclerosis (ALS4). Senataxin bears an RNA/DNA helicase domain suggesting a role in DNA and RNA processing and has recently been found to be involved in the DNA damage response. Alternate names for Senataxin include SEN1 homolog, SETX, ALS4, KIAA0625, SCAR1, and AOA2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Senataxin Antibody (NB100-57543)(2)

Publications using NB100-57543

• Zhao DY, Gish G, Braunschweig U et al. SMN and symmetric arginine dimethylation of RNA polymerase II C-terminal domain control termination. Nature. 2016-01-07 [PMID: 26700805] (IP, Chemotaxis, Human)
• Zhao D, Ni Z, Hu S et al. Regulation of transcription termination by FUS and TDP-43 bioRxiv 4373-01-01 (IP, Chemotaxis, Human)

Bioinformatics

• Gene Symbol: SETX
• Entrez:
  • 23064(Human)
• Uniprot:
  • Q7Z333(Human)