Simple Western lane view shows lysates of human brain (cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for Semaphorin 7A at approximately 109 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse ...read more
Western blot shows lysates of mouse brain tissue, mouse testis tissue, and mouse ovary tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Semaphorin 7A Antigen Affinity-purified Polyclonal Antibody ...read more
Detects mouse Semaphorin 7A in direct ELISAs and Western blots. In these formats, less than 1% cross‑reactivity with recombinant mouse (rm) Semaphorin 3C, rmSemaphorin 3E, rmSemaphorin 3F, rmSemaphorin 6A, rmSemaphorin 6B, rmSemaphorin 6C, recombinant human (rh) Semaphorin 3A, rhSemaphorin 3B, and rhSemaphorin 6D is observed.
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Semaphorin 7A (Sema7A, designated CD108, previously Sema K1 or Sema L), is an ~80 kDa membrane-anchored glycoprotein that is a member of the Semaphorin family of axon guidance molecules (1 - 4). On human erythrocytes, it is the John Milton Hagen (JMH) blood group antigen (4). Sema7A is the only known Class 7 or glycophosphatidylinositol (GPI)-linked semaphorin; its expression is concentrated in the brain, spleen and thymus (1 - 5). Mouse Sema7A cDNA encodes a 44 amino acid (aa) signal sequence, a 602 aa extracellular domain (ECD) including Sema and C2-type Ig-like domains, and an 18 aa propeptide/GPI membrane anchor signal sequence. Mature mouse Sema7A shares 89%, 98%, 85%, 86% and 89% aa identity with corresponding human, rat, bovine, canine and equine Sema7A, respectively. The Sema7A sema domain contains an RGD integrin interaction motif (4). Although it binds plexin-C1 in vitro and may be coexpressed with it, many of its activities depend on interaction with beta 1 integrins such as alpha 1 beta 1 (6 - 10). Sema7A signaling through the two receptors may cause opposing effects (8). Sema7A is an immune semaphorin, with expression and activity on CD4+CD8+ thymocytes, activated T cells, macrophages and microglia (2, 9 - 12). T cell Sema7A interacts with monocytic cells, stimulating their chemotaxis, production of pro-inflammatory cytokines, and dendritic differentiation (5, 6). However, on the T cells themselves, Sema7A downregulates TCR signaling by promoting TCR internalization, modulating T cell responses (9). In lung macrophages, Sema7A is induced by TGF-beta and participates in TGF-beta -induced lung fibrosis (12). Sema7A is also expressed on pre-osteoblasts and osteoclasts, where it promotes migration and fusion, respectively; on keratinocytes, where it promotes melanocyte spreading and dendricity; and on some neurons, for example, promoting axon outgrowth in the developing olfactory tract (8, 10, 13).
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This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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