Semaphorin 7A Antibody [Unconjugated]

Simple Western lane view shows lysates of human brain (cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for Semaphorin 7A at approximately 109 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse Semaphorin 7A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1835) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western blot shows lysates of mouse brain tissue, mouse testis tissue, and mouse ovary tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Semaphorin 7A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1835) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Semaphorin 7A at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Semaphorin 7A Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 7A
  Gln45-Ala646
  Accession # Q9QUR8
• Specificity: Detects mouse Semaphorin 7A in direct ELISAs and Western blots. In these formats, less than 1% cross‑reactivity with recombinant mouse (rm) Semaphorin 3C, rmSemaphorin 3E, rmSemaphorin 3F, rmSemaphorin 6A, rmSemaphorin 6B, rmSemaphorin 6C, recombinant human (rh) Semaphorin 3A, rhSemaphorin 3B, and rhSemaphorin 6D is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SEMA7A
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 2.5 ug/mL
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Semaphorin 7A Antibody [Unconjugated]

• CD108 antigen
• CD108
• CD108MGC126696
• CDw108
• H-SEMA-K1
• H-Sema-L
• JMH blood group antigen
• JMH
• John-Milton-Hargen human blood group Ag
• MGC126692
• sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin)7A (JMH blood group)
• sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin)7A
• sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, 7A
• Sema K1
• Sema L
• Sema7A
• SEMAK1
• SEMAL
• SEMALJohn Milton Hagen blood group H-Sema K1
• Semaphorin 7A
• semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group)
• semaphorin K1
• semaphorin L
• semaphorin-7A
• semaphorin-K1
• semaphorin-L

Background

Semaphorin 7A (Sema7A, designated CD108, previously Sema K1 or Sema L), is an ~80 kDa membrane-anchored glycoprotein that is a member of the Semaphorin family of axon guidance molecules (1 - 4). On human erythrocytes, it is the John Milton Hagen (JMH) blood group antigen (4). Sema7A is the only known Class 7 or glycophosphatidylinositol (GPI)-linked semaphorin; its expression is concentrated in the brain, spleen and thymus (1 - 5). Mouse Sema7A cDNA encodes a 44 amino acid (aa) signal sequence, a 602 aa extracellular domain (ECD) including Sema and C2-type Ig-like domains, and an 18 aa propeptide/GPI membrane anchor signal sequence. Mature mouse Sema7A shares 89%, 98%, 85%, 86% and 89% aa identity with corresponding human, rat, bovine, canine and equine Sema7A, respectively. The Sema7A sema domain contains an RGD integrin interaction motif (4). Although it binds plexin-C1 in vitro and may be coexpressed with it, many of its activities depend on interaction with beta 1 integrins such as alpha 1 beta 1 (6 - 10). Sema7A signaling through the two receptors may cause opposing effects (8). Sema7A is an immune semaphorin, with expression and activity on CD4⁺CD8⁺ thymocytes, activated T cells, macrophages and microglia (2, 9 - 12). T cell Sema7A interacts with monocytic cells, stimulating their chemotaxis, production of pro-inflammatory cytokines, and dendritic differentiation (5, 6). However, on the T cells themselves, Sema7A downregulates TCR signaling by promoting TCR internalization, modulating T cell responses (9). In lung macrophages, Sema7A is induced by TGF-beta and participates in TGF-beta -induced lung fibrosis (12). Sema7A is also expressed on pre-osteoblasts and osteoclasts, where it promotes migration and fusion, respectively; on keratinocytes, where it promotes melanocyte spreading and dendricity; and on some neurons, for example, promoting axon outgrowth in the developing olfactory tract (8, 10, 13).

1. Yazdani, U. and J.R. Terman (2006) Genome Biol. 7:211.
2. Kikutani, H. et al. (2007) Adv. Immunol. 93:121.
3. Sato, Y. and (1998) Biochim. Biophys. Acta 1443:419.
4. Yamada, A. et al. (1999) J. Immunol. 162:4094.
5. Holmes, S. et al. (2002) Scand. J. Immunol. 56:270.
6. Suzuki, K. et al. (2007) Nature 446:680.
7. Pasterkamp, R.J. et al. (2007) BMC Dev. Biol. 7:98.
8. Scott, G.A. et al. (2007) J. Invest. Dermatol. 128:151.
9. Czopik, A.K. et al. (2006) Immunity 24:591.
10. Pasterkamp, R.J. et al. (2003) Nature 424:398.
11. Mine, T. et al. (2000) Tissue Antigens 55:429.
12. Kang, H-R. et al. (2007) J. Exp. Med. 204:1083.
13. Delorme, G. et al. (2005) Biol. Cell 97:589.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Semaphorin 7A Antibody (AF1835)(3)

Publications using AF1835

• N Inoue, H Nishizumi, H Naritsuka, H Kiyonari, H Sakano Sema7A/PlxnCl signaling triggers activity-dependent olfactory synapse formation Nat Commun, 2018-05-09;9(1):1842. 2018-05-09 [PMID: 29743476] (IHC, Mouse)
• H Lee, LJ Macpherson, CA Parada, CS Zuker, NJP Ryba Rewiring the taste system Nature, 2017-08-09;548(7667):330-333. 2017-08-09 [PMID: 28792937] (IHC, Mouse)
• Parkash J, Messina A, Langlet F, Cimino I, Loyens A, Mazur D, Gallet S, Balland E, Malone S, Pralong F, Cagnoni G, Schellino R, De Marchis S, Mazzone M, Pasterkamp R, Tamagnone L, Prevot V, Giacobini P Semaphorin7A regulates neuroglial plasticity in the adult hypothalamic median eminence. Nat Commun, 2015-02-27;6(0):6385. 2015-02-27 [PMID: 25721933] (IHC, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: SEMA7A
• Uniprot:
  • Q9QUR8()