Semaphorin 6D Overexpression Lysate (Native)


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Product Details

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

Semaphorin 6D Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_705870. The protein contains a C-terminal DDK tag.
Homo sapiens SEMA6D, mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
111.3 kDa

Lysate Details for Semaphorin 6D

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Semaphorin 6D Overexpression Lysate (Native)

  • KIAA1479FLJ11598
  • sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D
  • Sema6D
  • semaphorin-6D


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Contact Information

Product PDFs


Gene Symbol SEMA6D

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