Semaphorin 4D/CD100 Antibody Pair [HRP]

Sandwich ELISA: Semaphorin 4D/CD100 Antibody Pair [HRP] [NBP2-79597] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP
• Standard Curve Range: 46.88-3000 pg/ml (example only; lot dependent)
• Sensitivity: 47 pg/ml (example only; lot dependent)

Semaphorin 4D/CD100 Antibody Pair [HRP] Summary

• Description: Solid Phase sandwich ELISA for the quantitative determination of Mouse Semaphorin 4D/CD100.
• Additional Information: Recombinant Monoclonal Antibody
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Mouse Semaphorin 4D/CD100.
• Source: HEK293
• Standard Curve Range: 46.88-3000 pg/ml (example only; lot dependent)
• Sensitivity: 47 pg/ml (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Sample Volume: 100 ul
• Gene: SEMA4D

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Semaphorin 4D/CD100 Antibody Pair [HRP]

• A8
• BB18
• CD100 antigen
• CD100
• CD100M-sema-G
• COLL-4
• FLJ33485
• FLJ34282
• FLJ39737
• FLJ46484
• immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4D
• MGC169138
• MGC169141
• M-sema-G
• sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4D
• SEMA4D
• SEMAJ
• Semaphorin 4D
• semaphorin-4D

Background

Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100, a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: SEMA4D